Antibacterial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth
Antibacterial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth
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DOI：
10.1016/j.lwt.2010.10.005
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发表时间：
2011-05-01
影响因子：
6
通讯作者：
Mazumdar, Anisha
中科院分区：
文献类型：
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作者：
Hussain, Abdullah I.;Anwar, Farooq;Mazumdar, Anisha
研究了六种兰科植物精油对致病菌和食品腐败菌的抑菌活性。采用气相色谱法和气相质谱法对精油的化学成分进行了分析，主要成分为：迷迭香中的1,8-桉叶醇（29.2%）、樟脑（17.2%）、α -蒎烯（11.5%）、香茅醛（20.5%）、-香叶醇（17.0%）、-香茅醇（11.5%）、香茉莉中的-香茅醇（11.5%）、鼠尾草中的1,8-桉叶醇（27.4%）、α -图琼（16.3%）、-图琼（11.2%）、龙脑（10.4%）、樟脑（7.98%）、香樟醇（25.1%）、香樟酸酯（22.5%）、香樟油（25.1%）、香樟油（22.5%）和香樟油（22.5%）。百里香中百里香酚（52.4%）、对伞花香（17.9%）、广藿香中胆烯醇（22.7%）、α -牛膝烯（17.1%）、α -鸟嘌呤（13.8%）。在定量的基础上，用标准纯化合物的标定曲线计算出精油中1,8-桉叶醇、香茅醛、1,8-桉叶醇、芳樟醇、百里香酚和广藿香醇的含量分别为28.4、19.0、26.7、23.3、51.1和21.1 g/100 g。采用改进的瑞祖灵微滴板法对其挥发油及其主要成分的抑菌活性进行了评价。所有被分析的精油对大多数被测试的菌株都有抑制作用。普通胸腺精油的抑制作用最强。结果表明，改进的瑞祖林法可以有效、可靠地评价精油对革兰氏阳性和阴性细菌群的抑菌活性。本研究结果还表明，具有较高抗菌活性的扇叶科植物精油通常富含氧合单萜类化合物。(C) 2010 Elsevier Ltd.版权所有。
The aim of this work was to investigate the antibacterial activity of six Lamiaceae essential oils, against pathogenic and food spoilage bacteria. The chemical profiles of essential oils were evaluated by the means of GC and GC MS. The major constituents of the oils were 1,8-cineol (29.2%), camphor (17.2%), alpha-pinene (11.5%) in Rosmarinus officinalis, citronellal (20.5%), beta- geraniol (17.0%), beta-citronellol (11.5%) in Melissa officinalis, 1,8-cineol (27.4%), alpha-thujone (16.3%), beta-thujone (11.2%), borneol (10.4%), camphor (7.98%) in Salvia officinalis, linalool (25.1%), linalyl acetate (22.5%) in Lavandula angustifolia, thymol (52.4%), p-cymene (17.9%) in Thymus vulgaris and Patcholene alcohol (22.7%), alpha-bulnesene (17.1%), alpha-guaine (13.8%) in Pogostemon cablin. On quantitative basis, the amounts of 1,8-cineol, citronellal, 1,8-cineol, linalool, thymol and patchouli alcohol, calculated using calibrated curve with pure standard compounds, in the respective essential oils were found to be 28.4, 19.0, 26.7, 23.3, 51.1 and 21.1 g/100 g of oil, respectively. The modified resazurin microtitre-plate assay was used to evaluate the antibacterial activity of the essential oils and their principal components. All the essential oils analyzed presented inhibitory effects on most of the strains tested. Thymus vulgaris essential oil showed the highest inhibition. It was concluded that modified resazurin assay could be effectively used for reliable assessment of antibacterial activity of the tested essential oils against several Gram positive and negative bacterial taxa. The present results also demonstrated that Lamiaceae essential oils exhibiting higher antibacterial activity were generally rich in oxygenated monoterpens. (C) 2010 Elsevier Ltd. All rights reserved.