Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation
Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation
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DOI：
10.1023/a:1011927004307
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发表时间：
2001-01-01
影响因子：
4
通讯作者：
Perneczky, A
中科院分区：
文献类型：
--
作者：
Demuth, T;Hopf, NJ;Perneczky, A
开发了一种新的迁移测定，即延时个体细胞迁移测定（TIM 测定），它允许在受控条件下观察细胞超过 24 小时。利用该技术，研究了 8 种人胶质母细胞瘤细胞系的体外迁移行为。特殊功能是同时记录单个细胞的迁移参数，即单个细胞的迁移速度和迁移路径。同一细胞系的细胞群的迁移速度范围为 0 至 24 mum/h。检查迁移路径是否具有方向性。三分之二的细胞表现出定向迁移。根据视觉判断，迁移路径进一步分类为线性、振荡或混合。迁移指数平均值为 91%。所提出的 TIM 测定可以评估几个新参数，这可能有助于识别具有不同生物学特征的神经胶质瘤亚组。
A new migration assay, the time-lapse individual cell migration assay (TIM-assay), was developed, which allows the observation of cells over 24 h under controlled conditions. Using this technique, the migratory behavior of 8 human glioblastoma cell lines in vitro was studied. Special features are simultaneous documentation of migratory parameters of individual cells, i.e., migration velocities and migration paths of individual cells. Migration velocity for cell populations of the same cell line ranged from 0 to 24 mum/h. The migration paths were examined for being directional. Two thirds of all cells showed directional migration. Migration paths were further classified according to visual judgements for being linear, oscillating or mixed. The migration index had a mean of 91%. The presented TIM-assay allows the assessment of several new parameters, that may be useful to identify subgroups of gliomas with different biological characteristics.