Induction of the soybean phytoalexins coumestrol and glyceollin by Aspergillus
Induction of the soybean phytoalexins coumestrol and glyceollin by Aspergillus
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DOI：
10.1021/jf9912809
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发表时间：
2000-06-01
影响因子：
6.1
通讯作者：
Cleveland, TE
中科院分区：
文献类型：
--
作者：
Boué, SM;Carter, CH;Cleveland, TE
已知大豆产生的几种植物抗毒素是雌激素，对人类健康有潜在的有益影响。大豆植物抗毒素产量的增加将促进这一领域的研究工作。本研究以大豆子叶为材料，利用曲霉A. sojae，大豆A.米A.尼日尔和A.黄的测试的所有四种曲霉菌都引起了活大豆子叶中植物防御素的积累。时间进程研究的结果表明，在接种A. sojae其他曲霉属物种导致大豆抗毒素的积累水平显着较低。从接种A.尼日尔.食品级A诱导的大豆植物抗毒素。sojae和A.该方法允许收集更高浓度的植物抗毒素，用于在几项体外和体内生物学研究中进行进一步检查，以确定潜在的雌激素活性。
Several isoflavonoid phytoalexins produced by soybeans are known to be estrogenic, with potential beneficial health effects in humans. Increased production of phytoalexins by the soybean plant will facilitate research efforts in this area. In this study, phytoalexin induction and accumulation in soybean cotyledon tissue was observed using four species of Aspergillus: A. sojae, A. oryzae, A. niger, and A. flavus. All four Aspergillus species tested elicited phytoalexin accumulation in living soybean cotyledons. Results from a time course study indicated that maximum concentrations of the phytoalexin glyceollin, 955 mu g/g fresh weight (fw), occurred at day 3 in soybean cotyledon tissue inoculated with A. sojae. Other Aspergillus species caused an accumulation of glyceollin at significantly lower levels. A maximum concentration of coumestrol of 27.2 mu g/g fw was obtained from soybean cotyledons inoculated with A. niger. Soybean phytoalexins induced by food-grade A. sojae and A. oryzae allowed the collection of higher concentrations of phytoalexins for further examination in several in vitro and in vivo biological studies conducted to determine potential estrogenic activities.