Inhibiting Roles of Berberine in Gut Movement of Rodents are Related to Activation of the Endogenous Opioid System
Inhibiting Roles of Berberine in Gut Movement of Rodents are Related to Activation of the Endogenous Opioid System
复制标题
小檗碱对啮齿动物肠道运动的抑制作用与内源性阿片系统的激活有关
DOI：
10.1002/ptr.4926
复制
发表时间：
2013-10-01
影响因子：
7.2
通讯作者：
Jia, Yijun
中科院分区：
文献类型：
--
作者：
Feng, Yajing;Li, Yongyu;Jia, Yijun
尽管小檗碱（BER）在治疗胃肠道（GI）疾病方面很受欢迎，但其机制尚不清楚。为了探讨BER对啮齿类动物胃肠道运动的影响及其可能机制，我们首先通过记录大鼠空肠和结肠的肌电活动以及用活性炭标记的小鼠上消化道转运来研究胃肠道运动。采用酶联免疫吸附法（ELISA）或放射免疫法（RIA）测定血浆胃泌素、胃动素、生长抑素和胰高血糖素样肽-1（Glp-1）水平。此外，内源性阿片肽（β-内啡肽，强啡肽-A，甲硫氨酸脑啡肽）的RIA检测BER处理后。结果表明，BER浓度依赖性地抑制肌电活动和胃肠道传输，阿片受体拮抗剂可不同程度地拮抗BER的抑制作用。阿片受体拮抗剂也能拮抗BER引起的血浆生长抑素和Glp-1升高，胃泌素和胃动素降低。此外，血浆β-内啡肽水平，但不强啡肽-A和甲硫氨酸脑啡肽，应用BER增加。综上所述，BER对胃肠道运动有抑制作用，对生长抑素、Glp-1有上调作用，对胃泌素、胃动素有下调作用。BER对胃肠道运动和胃肠道激素水平影响的药理机制与内源性阿片系统密切相关。版权所有（c）2013约翰威利父子有限公司
Although Berberine (BER) is popular in treating gastrointestinal (GI) disorders, its mechanisms are not clear yet. In order to investigate the effects and possible mechanism of BER on GI motility in rodents, we first explored GI motility by recording the myoelectrical activity of jejunum and colon in rats, and upper GI transit with a charcoal marker in mice. Then, the plasma levels of gastrin, motilin, somatostatin and glucagon-like-peptide-1 (Glp-1) were measured by ELISA or radioimmunoassay (RIA). Furthermore, endogenous opioid-peptides (-endorphin, dynorphin-A, met-enkephalin) were detected by RIA after treatment with BER. Our results showed that BER concentration-dependently inhibited myoelectrical activity and GI transit, which can be antagonized by opioid-receptor antagonists to different extents. The elevated somatostatin and Glp-1, and decreased gastrin and motilin in plasma, which were caused by BER application, also could be antagonized by the opioid-receptor antagonists. Additionally, plasma level of -endorphin, but not dynorphin-A and met-enkephalin, was increased by applying BER. Taken together, these studies show that BER plays inhibiting roles on GI motility and up-regulating roles on somatostatin, Glp-1 and down-regulating roles on gastrin, motilin. The pharmacological mechanisms of BER on GI motility and plasma levels of GI hormones were discovered to be closely related to endogenous opioid system. Copyright (c) 2013 John Wiley & Sons, Ltd.