Characterization of a β-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose
Characterization of a β-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose
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DOI：
10.1016/j.jbiotec.2007.07.939
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发表时间：
2007-10-15
影响因子：
4.1
通讯作者：
Fernández-Lobato, Maria
中科院分区：
文献类型：
--
作者：
Alvaro-Benito, Miguel;de Abreu, Miguel;Fernández-Lobato, Maria
β-呋喃果糖苷酶是工业生物技术中的有力工具。我们的特点是胞外P-呋喃果糖苷酶从酵母Schwanniomyces occluentalis。该酶具有广泛的底物特异性，水解蔗糖、1-蔗果三糖、蔗果三糖和棉子糖，具有不同的催化效率（k（cat）/K-m）。尽管该酶催化的主要反应是蔗糖水解，但它也通过转果糖基化产生两种低聚果糖（FOS）。H-1、C-13和2D-NMR技术的组合表明，主要产物是益生元三糖6-蔗果三糖。用该β-呋喃果糖苷酶获得的6-蔗果三糖产率，对于我们的关注，高于用其它6-蔗果三糖产生酶报道的那些产率，在动力学最大值（76 g I-1）和反应平衡（44 g I-1）下都是如此。在动力学最大值下的总FOS产量为101 g I-1，其对应于反应混合物中总碳水化合物的16.4%（w/w）。(C)2007 Elsevier B. V.保留所有权利。
beta-Fructofuranosidases are powerful tools in industrial biotechnology. We have characterized an extracellular P-fructofuranosidase from the yeast Schwanniomyces occidentalis. The enzyme shows broad substrate specificity, hydrolyzing sucrose, 1-kestose, nystose and raffinose, with different catalytic efficiencies (k(cat)/K-m). Although the main reaction catalysed by this enzyme is sucrose hydrolysis, it also produces two fructooligosaccharides (FOS) by transfructosylation. A combination of H-1, C-13 and 2D-NMR techniques shows that the major product is the prebiotic trisaccharide 6-kestose. The 6-kestose yield obtained with this beta-fructofuranosidase is, to our concern, higher than those reported with other 6-kestose-producing enzymes, both at the kinetic maximum (76 g I-1) and at reaction equilibrium (44 g I-1). The total FOS production in the kinetic maximum was 101 g I-1, which corresponded to 16.4% (w/w) referred to the total carbohydrates in the reaction mixture. (C) 2007 Elsevier B.V. All rights reserved.