Proximal tubule volume regulation in hyperosmotic media: intracellular K+, Na+, and Cl-.
Proximal tubule volume regulation in hyperosmotic media: intracellular K+, Na+, and Cl-.
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高渗介质中的近端小管容量调节：细胞内 K、Na 和 Cl-。
DOI：
10.1152/ajpcell.1989.257.6.c1093
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Lechene,C
中科院分区：
文献类型：
--
作者：
Rome,L;Grantham,J;Savin,V;Lohr,J;Lechene,C
来自兔肾皮质的非灌注近端S2节段已显示在介质渗透压缓慢升高时保持细胞体积恒定，但如果介质渗透压突然升高，则收缩并且不表现出调节体积增加（RVI）（J. Lohr和J. Grantham. J. Clin. Invest. 78：1165-1172，1986）。在本研究中，培养基中的0.5 mM丁酸盐1）将范围从361 mosmol/kgH 2 O扩展至450 mosmol/kgH 2 O，在此范围内，随着渗透压摩尔浓度逐渐升高，细胞保持体积恒定，2）当渗透压摩尔浓度从295 mosmol/kgH 2 O快速升高至400 mosmol/kgH 2 O时，细胞收缩后恢复RVI。容量调节与细胞内Na+和Cl-的净增加有关，但K+无变化（通过电子探针测量）。Na+和Cl-的增量不足以解释体积维持或恢复所需的渗透剂的总添加量。逐步和快速渗透压变化的细胞内渗透压摩尔预期增加的分数占[（K+）i +（Na+）i +（Cl-）i]的增加分别为52%和21%。我们得出结论，丁酸盐通过促进Na+和Cl-的加入以及其他破坏因素来增强S2段在高渗介质中调节体积的能力。
Nonperfused proximal S2 segments from rabbit kidney cortex have been shown to keep cell volume constant as medium osmolality is slowly raised but to shrink and not exhibit regulatory volume increase (RVI) if medium osmolality is abruptly elevated (J. Lohr and J. Grantham. J. Clin. Invest. 78: 1165-1172, 1986). In the current study, 0.5 mM butyrate in the medium 1) extended the range from 361 to 450 mosmol/kgH2O over which cells maintained volume constant as osmolality was gradually raised and 2) restored RVI after cell shrinkage when osmolality was rapidly raised from 295 to 400 mosmol/kgH2O. Volume regulation was associated with net increases in intracellular Na+ and Cl- but no change in K+ (measured by electron probe). The increments in Na+ and Cl- were insufficient to account for the total addition of osmolytes required for volume maintenance or restoration. The fraction of the expected increase in intracellular osmoles accounted for by the increase in [(K+)i + (Na+)i + (Cl-)i] was 52 and 21% for gradual and rapid osmotic changes, respectively. We conclude that butyrate enhances the capacity of S2 segments to regulate volume in hyperosmotic medium by promoting addition of Na+ and Cl- and by other undermined factors.
培养的大鼠近端小管细胞中 Na-H 交换和 Na-K 泵活性的耦合。
DOI：
10.1152/ajpcell.1986.251.5.c815
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Harris,RC;Seifter,JL;Lechene,C
通讯作者：
Lechene,C
细胞体积和细胞质凝胶
影响因子：
2.7
作者：
C. Lechene
通讯作者：
C. Lechene
肾内髓质中具有渗透活性的有机溶质。
影响因子：
19.6
作者：
R. Balaban;M. Burg
通讯作者：
M. Burg