Calcite-Assisted Localization and Kinetics (CLocK) Microscopy
Calcite-Assisted Localization and Kinetics (CLocK) Microscopy
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DOI：
10.1021/acs.jpclett.2c03028
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发表时间：
2022-11-07
影响因子：
5.7
通讯作者：
Willets，Katherine A.
中科院分区：
文献类型：
--
作者：
Monaghan，Joseph W.;O'Dell，Zachary J.;Willets，Katherine A.
基于定位的超分辨率成像技术已经将光学显微镜的空间分辨率提高到远低于衍射极限，但将额外的信息编码到超分辨率图像中，例如各向异性和取向，仍然是一个挑战。在这里，我们介绍方解石辅助定位和动力学（CLocK）显微镜，多参数超分辨率成像技术很容易集成到任何现有的光学显微镜设置，成本低，分析简单。通过将旋转的方解石晶体放置在光学显微镜的无限空间中，ClockK显微镜提供了即时的偏振和取向信息，同时保持了以<10 nm的分辨率定位发射体/散射体的能力。此外，比相机的积分时间短一个数量级的动力学信息被编码在Clock图像的独特点扩散函数中，从而允许对动态过程（例如单纳米颗粒溶解和单分子表面增强拉曼散射）的新的机理性洞察。
Localization-based super-resolution imaging techniques have improved the spatial resolution of optical microscopy well below the diffraction limit, yet encoding additional information into super-resolved images, such as anisotropy and orientation, remains a challenge. Here we introduce calcite-assisted localization and kinetics (CLocK) microscopy, a multiparameter super-resolution imaging technique easily integrated into any existing optical microscope setup at low cost and with straightforward analysis. By placing a rotating calcite crystal in the infinity space of an optical microscope, CLocK microscopy provides immediate polarization and orientation information while maintaining the ability to localize an emitter/scatterer with <10 nm resolution. Further, kinetic information an order of magnitude shorter than the integration time of the camera is encoded in the unique point spread function of a CLocK image, allowing for new mechanistic insight into dynamic processes such as single-nanoparticle dissolution and single-molecule surface-enhanced Raman scattering.