Dancing Styles of Collective Cell Migration: Image-Based Computational Analysis of JRAB/MICAL-L2.
Dancing Styles of Collective Cell Migration: Image-Based Computational Analysis of JRAB/MICAL-L2.
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DOI：
10.3389/fcell.2018.00004
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发表时间：
2018
影响因子：
5.5
通讯作者：
Sasaki T
中科院分区：
文献类型：
--
作者：
Sakane A;Yoshizawa S;Yokota H;Sasaki T
在形态发生、血管生成和伤口愈合过程中可以观察到集体细胞迁移，这种类型的细胞迁移也有助于某些癌症的有效转移。因为集体迁移的细胞比单个细胞的随机组合组织得更好，所以在迁移集群中似乎有一种秩序。广泛的研究已经确定了大量参与集体细胞迁移的分子，这些因素已经使用成像技术的巨大进步进行了分析。然而，到目前为止，还不清楚无数细胞是如何整合成一个单一的单位的。最近，我们观察到由于JRAB/MICAL-L2构象变化的损害，导致了不平衡的集体细胞迁移，这种迁移可以被比喻为精确舞蹈或awa-odori，这是日本传统舞蹈，类似于里约热内卢狂欢节的风格。本文首先简要介绍了基于图像的细胞迁移计算分析的历史，解释了为什么对集体细胞行为的风格化进行定量分析是困难的，最后介绍了我们最近在JRAB/MICAL-L2上的工作，这是将细胞生物学、实时成像和计算生物学相结合的多学科方法的成功例子。结合起来，这些方法可以对集体细胞迁移的“舞蹈风格”进行定量评估。
Collective cell migration is observed during morphogenesis, angiogenesis, and wound healing, and this type of cell migration also contributes to efficient metastasis in some kinds of cancers. Because collectively migrating cells are much better organized than a random assemblage of individual cells, there seems to be a kind of order in migrating clusters. Extensive research has identified a large number of molecules involved in collective cell migration, and these factors have been analyzed using dramatic advances in imaging technology. To date, however, it remains unclear how myriad cells are integrated as a single unit. Recently, we observed unbalanced collective cell migrations that can be likened to either precision dancing or awa-odori, Japanese traditional dancing similar to the style at Rio Carnival, caused by the impairment of the conformational change of JRAB/MICAL-L2. This review begins with a brief history of image-based computational analyses on cell migration, explains why quantitative analysis of the stylization of collective cell behavior is difficult, and finally introduces our recent work on JRAB/MICAL-L2 as a successful example of the multidisciplinary approach combining cell biology, live imaging, and computational biology. In combination, these methods have enabled quantitative evaluations of the “dancing style” of collective cell migration.