Mirodenafil Prevents Bladder Dysfunction Induced by Chronic Bladder Ischemia in Rats
Mirodenafil Prevents Bladder Dysfunction Induced by Chronic Bladder Ischemia in Rats
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DOI：
10.5213/inj.2015.19.1.19
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发表时间：
2015-03-01
影响因子：
2.3
通讯作者：
Lee, Jeong Gu
中科院分区：
文献类型：
--
作者：
Choi, Hoon;Bae, Jae Hyun;Lee, Jeong Gu
目的：探讨米罗地那非对慢性膀胱缺血模型大鼠膀胱功能的保护作用。方法：24只SD大鼠随机分为3组：假手术对照组(对照组)、脑缺血模型组(脑损伤模型组)和脑损伤大鼠每日灌胃4 mg/kg米罗地那非(脑缺血+米罗地那非)组。CBI组和CBI+米罗地那非组造成髂动脉内皮损伤，损伤后给予2%的胆固醇饮食。手术后四周，CBI+米罗地那非组开始每日服用米罗地那非，为期四周。结果：与对照组和CBI+米罗地那非组比较，CBI组大鼠排尿频率明显增加，膀胱容量降低，顺应性降低。逼尿肌条研究表明，CBI组的卡巴胆碱引起的收缩反应的幅度明显低于对照组或CBI+米罗地那非组。与未经治疗的CBI大鼠相比，在CBI诱导后每日添加米罗地那非可降低收缩反应。结论：米罗地那非对脑损伤所致的大鼠膀胱功能障碍具有保护作用。
Purpose: To investigate the protective effect of mirodenafil on bladder function in a rat model of chronic bladder ischemia CBI).Methods: Twenty-four Sprague-Dawley rats were randomized to three groups: untreated, sham-operated rats control group); untreated, CBI model rats CBI group); and CBI rats treated daily with 4 mg/kg mirodenafil CBI + mirodenafil group). The CBI and CBI + mirodenafil groups underwent endothelial injury to the iliac arteries and were fed a 2% cholesterol diet after injury. Four weeks after surgery, the CBI + mirodenafil group started daily treatment with mirodenafil for four weeks. Eight weeks after surgery, continuous in vivo cystometry and in vitro organ bath studies of detrusor muscle strips were performed.Results: In vivo cystometry revealed that the rats in the CBI group had a significantly higher micturition frequency, lower bladder capacity, and lower compliance than the rats in the control and CBI + mirodenafil groups. The detrusor muscle strip study showed that the magnitude of the carbachol-induced contractile response was significantly lower in the CBI group compared to either the control or CBI + mirodenafil group. Addition of daily mirodenafil after induction of CBI decreased the contractile response, compared to untreated CBI rats. CBI induced submucosal fibrosis and degenerative changes in bladder walls, which was reversed by the addition of mirodenafil.Conclusions: Daily treatment with mirodenafil showed protective effects against bladder dysfunction resulting from CBI in rats.