Analysis of Protein Stability by the Cycloheximide Chase Assay.
Analysis of Protein Stability by the Cycloheximide Chase Assay.
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DOI：
10.21769/bioprotoc.1374
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发表时间：
2015-01-05
期刊：
影响因子：
0.8
通讯作者：
Yang, Pan-Chyr
中科院分区：
文献类型：
--
作者：
Kao, Shih-Han;Wang, Wen-Lung;Yang, Pan-Chyr
放线菌酮是一种蛋白质生物合成的抑制剂，由于它阻止了翻译延伸，因此已经比较了真核细胞中蛋白质的稳定性。它被广泛用于细胞生物学中确定给定蛋白质的半衰期，并在癌症研究中获得了很大的普及。在这里，我们提出了一个完整的放线菌酮追逐试验在我们的实验室使用肺腺癌细胞系，CL 1 -5，作为一个模型。
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.