Effects of concentrated fine ambient particles on rat plasma levels of asymmetric dimethylarginine
Effects of concentrated fine ambient particles on rat plasma levels of asymmetric dimethylarginine
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DOI：
10.1080/08958370490439678
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发表时间：
2004-06-01
影响因子：
2.1
通讯作者：
Harkema, JR
中科院分区：
文献类型：
--
作者：
Dvonch, JT;Brook, RD;Harkema, JR
环境细颗粒物（PM2.5）对健康的影响及其对血管内皮功能的潜在影响尚未得到深入研究。由于内皮功能障碍在动脉粥样硬化及其并发症的发病机制中起着重要作用，我们在一项初步研究中检测了高浓度环境细颗粒（CAPs）对不对称二甲基精氨酸（ADMA）血浆水平的影响。ADMA是一种循环内源性一氧化氮合酶（NOS）抑制剂，与血管功能受损和心血管事件风险增加有关。一个移动空气研究实验室（AirCARE 1）被用来为这项在密歇根州底特律进行的研究提供“真实世界”的cap暴露。2002年7月，14只布朗挪威大鼠连续3天（每天8小时）暴露于过滤空气（FA） （n=7）或cap (0.1-2.5 mum) （n=7）。在此期间，大鼠暴露于平均颗粒质量浓度为354马克杯/米(3)。暴露24 h后采集大鼠血浆样本。与FA暴露的大鼠相比，cap暴露的大鼠血浆ADMA浓度显著升高（平均+/-标准差=1.49+/-0.18比1.29+/-0.26，单尾t检验p= 0.05）。气象资料和大气颗粒物微量元素组成分析表明，局地颗粒物排放源对大气颗粒物总质量的贡献很大。这项初步研究的结果表明，暴露于高浓度的PM2.5可能引发循环ADMA水平的急性增加。这一发现对颗粒污染物对血管舒缩张力的调节和不良心血管事件的倾向具有启示意义。
The health effects of ambient fine particulate matter (PM2.5) and its potential impact on vascular endothelial function have not been thoroughly investigated. As endothelial dysfunction plays an important role in the pathogenesis of atherosclerosis and its complications, we examined the effects of concentrated fine ambient particles (CAPs) on the plasma level of asymmetric dimethylarginine (ADMA) in a pilot study. ADMA is a circulating endogenous inhibitor of nitric oxide synthase (NOS) that is associated with impaired vascular function and increased risk for cardiovascular events. A mobile air research laboratory (AirCARE 1) was used to provide "real-world" CAPs exposures for this study conducted in Detroit, MI. Fourteen Brown Norway rats were exposed to filtered air (FA) (n=7) or CAPs (0.1-2.5 mum) (n=7) for 3 consecutive days (8 h/day) in July 2002. Rats were exposed during these periods to average particle mass concentrations of 354 mug/m(3). Rat plasma samples were collected 24 h postexposure. Plasma concentrations of ADMA were significantly elevated in rats exposed to CAPs versus those exposed to FA (mean+/-standard deviation=1.49+/-0.18 vs. 1.29+/-0.26 muM, p=.05 by one-tailed t-test). Analyses of meteorological data and CAPs trace element composition suggest that local particle emission sources contributed largely to overall mass of CAPs. Results of this pilot study suggest that exposure to PM2.5 at high concentrations may trigger an acute increase in circulating ADMA level. This finding has implications for the regulation of vasomotor tone by particulate pollutants and the propensity for adverse cardiovascular events.