Expression of PSA-NCAM and synaptic proteins in the amygdala of psychiatric disorder patients
Expression of PSA-NCAM and synaptic proteins in the amygdala of psychiatric disorder patients
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DOI：
10.1016/j.jpsychires.2011.10.011
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发表时间：
2012-02-01
影响因子：
4.8
通讯作者：
Nacher, Juan
中科院分区：
文献类型：
--
作者：
Varea, Emilio;Guirado, Ramon;Nacher, Juan
神经影像学揭示了不同精神疾病的杏仁核结构异常。多唾液酸化神经细胞粘附分子（PSA-NCAM）是一种与神经元结构可塑性相关的分子，其表达在精神分裂症、重度抑郁症和这些疾病的动物模型中发生改变，可能参与了这些变化。然而，PSA-NCAM 尚未在人类杏仁核中进行研究。为了了解其表达和突触前标记物的表达是否受到精神疾病的影响，我们分析了斯坦利神经病理学联盟的死后切片，其中包括对照组、精神分裂症、双相情感障碍和重度抑郁症患者。 PSA-NCAM 在神经元胞体和神经网点中表达，其中许多对应于中间神经元。抑郁症患者的基底外侧和基底内侧杏仁核中 PSA-NCAM 表达降低；在不同的细胞核中，突触素和 GAD67 也减少，而 VGLUT-1 增加。在双相情感障碍患者的外侧核中发现 PSA-NCAM 表达增加；在基底外侧核和外侧核中，突触素和 GAD67 减少，VGLUT-1 增加。精神分裂症患者基底外侧核突触素和GAD67的表达下调。这些结果表明，抑制性和兴奋性杏仁核回路在这些疾病中受到影响，并且抑郁症和双相情感障碍患者中 PSA-NCAM 表达异常可能是这些改变的基础。 (C) 2011 Elsevier Ltd. 保留所有权利。
Neuroimaging has revealed structural abnormalities in the amygdala of different psychiatric disorders. The polysialylated neural cell adhesion molecule (PSA-NCAM), a molecule related to neuronal structural plasticity, which expression is altered in schizophrenia, major depression and in animal models of these disorders, may participate in these changes. However, PSA-NCAM has not been studied in the human amygdala. To know whether its expression and that of presynaptic markers, was affected in psychiatric disorders, we have analyzed post-mortem sections from the Stanley Neuropathology Consortium, which includes controls, schizophrenia, bipolar and major depression patients. PSA-NCAM was expressed in neuronal somata and neuropil puncta, many of which corresponded to interneurons. Depressed patients showed decreases in PSA-NCAM expression in the basolateral and basomedial amygdala; synaptophysin and GAD67 were also decreased, while VGLUT-1 was increased, in different nuclei. Increases in PSA-NCAM expression were found in the lateral nucleus of bipolar patients; synaptophysin and GAD67 were reduced, and VGLUT-1 increased, in their basolateral and lateral nuclei. The expression of synaptophysin and GAD67 was downregulated in the basolateral nucleus of schizophrenics. These results indicate that inhibitory and excitatory amygdaloid circuits are affected in these disorders and that abnormal PSA-NCAM expression in depressive and bipolar patients may underlie these alterations. (C) 2011 Elsevier Ltd. All rights reserved.