Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase
复制标题
血小板内皮聚集受体-1通过整合素β-1和粘着斑激酶调节牛肌肉卫星细胞迁移和分化
DOI：
10.1080/19336918.2019.1619434
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发表时间：
2019-01-01
影响因子：
3.2
通讯作者：
Tong, Huili
中科院分区：
文献类型：
--
作者：
Pang, Yusheng;Zhang, Ziheng;Tong, Huili
摘要PEAR1在牛MDSC分化过程中高度表达。然而，其生物学功能仍不清楚。Western blotting结果显示PEAR 1在细胞分化的第0天和第2天之间增加，从第3天开始减少。此外，划痕试验表明，PEAR 1过表达后伤口愈合率增加，而其抑制后伤口愈合率下降。同时，我们发现，在PEAR 1诱导后，粘着斑相关蛋白和MyoG的表达以及肌管融合率均增加。然而，当PEAR1被抑制时，得到相反的结果。免疫沉淀显示PEAR 1和ITGB 1之间的关联。值得注意的是，FAK和ITGB1的抑制抑制了细胞分化。总之，我们的研究表明PEAR 1参与了牛MDSC迁移和分化的调节。
ABSTRACT PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.