The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity
The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity
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DOI：
10.1093/molbev/msg088
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发表时间：
2003-05-01
影响因子：
10.7
通讯作者：
Bailey, PC
中科院分区：
文献类型：
--
作者：
Heim, MA;Jakoby, M;Bailey, PC
碱性螺旋-环-螺旋（bHLH）转录因子（TF）属于转录调节因子家族，存在于真核生物的三个王国中。这些蛋白质在动物中具有许多不同的功能，包括控制细胞增殖和特定细胞谱系的发育。它们控制基因转录的机制通常涉及同源二聚化或异源二聚化。在植物中，对bHLH家族知之甚少，但我们已经确定在拟南芥中有133个bHLH基因，并证实其中至少有113个表达。AtbHLH基因是A. thaliana的成员比大多数动物物种中发现的成员要多得多，与脊椎动物中的成员数量相当。与动物序列的比较表明，大多数植物bHLH基因进化的祖先组B类bHLH基因。通过对AtbHLH基因的研究，共鉴定出12个亚家族。在这些主要组中的每一个内，在DNA结合结构域之外存在保守的氨基酸序列基序。潜在的基因冗余较小的亚组的成员之间进行了分析，所得到的信息提供了一个简化的视觉解释的基因家族，确定相关的基因可能共享相似的功能。基于目前有限数量的植物bHLH蛋白的表征，我们预测，这个家庭的TF在植物细胞和组织发育以及植物代谢中具有一系列不同的作用。
Basic helix-loop-helix (bHLH) transcription factors (TFs) belong to a family of transcriptional regulators present in three eukaryotic kingdoms. Many different functions have been identified for these proteins in animals, including the control of cell proliferation and development of specific cell lineages. Their mechanism for controlling gene transcription often involves homodimerization or heterodimerization. In plants, little is known about the bHLH family, but we have determined that there are 133 bHLH genes in Arabidopsis thaliana and have confirmed that at least 113 of them are expressed. The AtbHLH genes constitute one of the largest families of transcription factors in A. thaliana with significantly more members than are found in most animal species and about an equivalent number to those in vertebrates. Comparisons with animal sequences suggest that the majority of plant bHLH genes have evolved from the ancestral group B class of bHLH genes. By studying the AtbHLH genes collectively, twelve subfamilies have been identified. Within each of these main groups, there are conserved amino acid sequence motifs outside the DNA binding domain. Potential gene redundancy among members of smaller subgroups has been analyzed, and the resulting information is presented to provide a simplified visual interpretation of the gene family, identifying related genes that are likely to share similar functions. Based on the current characterization of a limited number of plant bHLH proteins, we predict that this family of TFs has a range of different roles in plant cell and tissue development as well as plant metabolism.