Cultured versus freshly isolated adipose-derived stem cells in improvement of the histopathological outcomes in HCL-induced cystitis in a rat model
Cultured versus freshly isolated adipose-derived stem cells in improvement of the histopathological outcomes in HCL-induced cystitis in a rat model
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DOI：
10.1016/j.biopha.2022.113422
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发表时间：
2022-07-19
影响因子：
7.5
通讯作者：
Tanaka，Ryou
中科院分区：
文献类型：
--
作者：
Hendawy，Hanan;Metwally，Elsayed;Tanaka，Ryou
间质性膀胱炎(IC)是一种不治之症。IC的病因尚不清楚，也没有建立有效的治疗方法。在此，使用氯化氢(HCL)诱导的大鼠IC模型，研究了基质血管成分(SVF)和脂肪来源干细胞(ADSCs)的治疗效果。36只雌性SD大鼠随机分为4组：假手术组、HCL组、(HCL+SVF)组和(HCL+ADSCs)组，每组9只。模型组大鼠经尿道注入盐酸复制膀胱炎模型，假手术组用PBS溶液灌胃。HCl诱导IC组大鼠膀胱粘膜下层一次性注射SVF或ADSCs。对膀胱组织进行甲苯胺蓝、Masson三色、CD3和CD34检测，分别评价肥大细胞活化、纤维化、炎症细胞和膀胱再生情况。与HCl诱导的IC相比，膀胱内注射SVF或ADSCs显著减少肥大细胞的浸润、T细胞的激活和纤维化。综上所述，给予SVF细胞或培养的ADSCs以时间依赖的方式改善HCl诱导的膀胱损伤的组织病理学结果。值得注意的是，膀胱粘膜下层注射SVF被认为具有最有效的治疗效果，并可能成为未来临床应用的重要组成部分。
Interstitial cystitis (IC) is an incurable chronic disease. The etiology of IC is unclear, and no effective therapies have been established. Here, using a hydrogen chloride (HCL)-induced IC in a rat model, the therapeutic potency of stromal vascular fraction (SVF) and Adipose-derived stem cells (ADSCs) was studied. Thirty-six female Sprague Dawley rats were divided into four groups: the sham, HCL, (HCL+SVF) group, and (HCL+ADSCs) group (9 for each). Cystitis was induced by transurethral instillation of HCL, while PBS was used for the sham group. A single dose of SVF or ADSCs was injected into the submucosa of the rat bladder in HCL-induced IC groups. The bladder tissues were analyzed for Toluidine Blue, Masson Trichrome, CD3, and CD34 to evaluate mast cell activation, fibrosis, inflammatory cells, and bladder regeneration, respectively. Compared to HCL-induced IC, SVF or ADSCs injection into IC bladder dramatically decreased mast cell infiltration, T-cell activation, and fibrosis. Taken together, administration of SVF cells or cultured ADSCs improves the histopathological outcomes of HCL-induced bladder injury in a time-dependent manner. Of note, SVF injection into the bladder submucosa was estimated to have the most potent therapeutic efficacy and may represent an essential component in future clinical applications.