Molecular dissection of mitotic recombination in the yeast Saccharomyces cerevisiae
Molecular dissection of mitotic recombination in the yeast Saccharomyces cerevisiae
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DOI：
10.1128/mcb.23.4.1403-1417.2003
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发表时间：
2003-02-01
影响因子：
5.3
通讯作者：
Kupiec, M
中科院分区：
文献类型：
--
作者：
Aylon, Y;Liefshitz, B;Kupiec, M
在所有的真核生物中，染色体断裂的修复过程中起着重要的作用。我们对有丝分裂重组进行了系统的研究。使用几种检测方法，我们建立了修复单个双链断裂所需的事件的时间顺序。一旦染色体断裂，酵母细胞立即进行重组修复。在一小时内完成了分解，并显示出两个动力学间隙。通过使用这个动力学框架，我们还表征了几种蛋白质在重组过程中所起的作用。在没有Rad52的情况下，断裂的染色体末端，5'和3'，都迅速降解。这不是由于不能重组，因为3'单链DNA末端在缺乏供体序列的菌株中是稳定的。Rad57是两个连续的链交换反应所必需的。令人惊讶的是，我们发现Srs2解旋酶在重组过程中也起着早期的积极作用。
Recombination plays a central role in the repair of broken chromosomes in all eukaryotes. We carried out a systematic study of mitotic recombination. Using several assays, we established the chronological sequence of events necessary to repair a single double-strand break. Once a chromosome is broken, yeast cells become immediately committed to recombinational repair. Recombination is completed within an hour and exhibits two kinetic gaps. By using this kinetic framework we also characterized the role played by several proteins in the recombinational process. In the absence of Rad52, the broken chromosome ends, both 5' and 3', are rapidly degraded. This is not due to the inability to recombine, since the 3' single-stranded DNA ends are stable in a strain lacking donor sequences. Rad57 is required for two consecutive strand exchange reactions. Surprisingly, we found that the Srs2 helicase also plays an early positive role in the recombination process.