Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia
Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia
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DOI：
10.1002/jmv.20905
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发表时间：
2007-08-01
影响因子：
12.7
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Lorenzo, Felipe R.;Tsatsralt-Od, Bira;Okamoto, Hiroaki
虽然蒙古没有报告戊型肝炎暴发，但相当大比例的普通人群有戊型肝炎病毒(HEV)抗体。为了调查猪是否可能是蒙古HEV的宿主，对蒙古首都乌兰巴托周围四个养猪场的243头2个月或3个月大的猪的血清样本进行了抗HEV抗体和HEV RNA的检测。总体而言，223头猪(91.8%)的抗-HEV检测呈阳性，而89头猪(36.6%)可检测到HEV RNA。从病毒病猪分离到的89株HEV毒株在开放阅读框2的412个核苷酸(NT)序列中，彼此的同源性为78.7-100%，与原型3型HEV分离物(US1)的同源性为80.9-85.9%。在3型内，它们被划分为两个新的系统发育群，差异16.4-21.3%。分别代表基因3中的两个簇的swMN06-A1288和swMN06-C1056分离株的基因组长度分别为7,222和7,223个核苷酸(NT)，不包括聚(A)尾，在整个基因组中只有81.6%的同源性。与已报道的25株3型戊型肝炎病毒全基因组分离株相比，swMN06-A1288的同源性仅为84.9%，而swMN06-C1056的同源性仅为85.9%。系统发育分析证实了蒙古猪分离株与迄今报道的3型HEV分离株的远亲关系。这些结果表明，蒙古的农场猪经常感染可能是本土HEV 3型毒株，并可能是蒙古人感染HEV的来源之一。
Although no outbreaks of hepatitis E have been reported in Mongolia, a significant proportion of the general population had antibodies to hepatitis E virus (HEV). To investigate whether pigs are possible reservoirs of HEV in Mongolia, serum samples obtained from 243 2- or 3-month-old pigs on four swine farms surrounding Ulaanbaatar, the capital city of Mongolia, were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 223 pigs (91.8%) tested positive for anti-HEV, while 89 pigs (36.6%) had detectable HEV RNA. The 89 HEV isolates obtained from the viremic pigs were 78.7-100% identical to each other, and 80.9-85.9% similar to the prototype genotype 3 HEV isolate (US1) in the 412-nucleotide (nt) sequence within open reading frame 2. They were classified into two novel phylogenetic groups within genotype 3, differing by 16.4-21.3%. The swMN06-A1288 and swMN06-C1056 isolates, representing each of the two clusters within genotype 3, had a genomic length of nucleotides (nt) 7,222 nt and 7,223 nt, respectively, excluding the poly(A) tail, and shared only 81.6% over the entire genome. Upon comparison with the 25-reported genotype 3 HEV isolates over the entire genome, swMN06-A1288 had identities of merely up to 84.9%, while swMN06-C1056 of only up to 85.9%. Phylogenetic analysis confirmed the remote relatedness of the Mongolian swine isolates to the genotype 3 HEV isolates reported thus far. These results indicate that farm pigs in Mongolia are frequently infected with presumably indigenous HEV strains of genotype 3 and could be a source of HEV infections in humans in Mongolia.