Longitudinal selenium status in healthy British adults: assessment using biochemical and molecular biomarkers.
Longitudinal selenium status in healthy British adults: assessment using biochemical and molecular biomarkers.
复制标题
健康英国成年人的纵向硒状况：使用生化和分子生物标志物进行评估。
DOI：
10.1017/s0007114508006831
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Gordon,MichaelH
中科院分区：
文献类型：
--
作者：
Sunde,RogerA;Paterson,Elaine;Evenson,JacquelineK;Barnes,KimberlyM;Lovegrove,JulieA;Gordon,MichaelH
目前，人体硒（Se）需要量是基于硒状态的生化标志物。在大鼠中，组织谷胱甘肽过氧化物酶-1（Gpx 1）的mRNA水平可以有效地用于确定硒的需要量;血液Gpx 1 mRNA水平在硒缺乏大鼠中下降，因此基于分子生物学的标记物具有用于人类营养评估的潜力。为了研究分子生物学标记物评估人体硒状态的有效性，我们对英国阅读的39名受试者（年龄45 ± 11岁）进行了纵向研究。在第2、8、17和23周从每个受试者获得饮食日记（5天）和血液，并测定血浆硒、谷胱甘肽过氧化物酶（Gpx 3）酶活性和硒蛋白mRNA水平。没有显着的纵向影响硒生物标志物。硒摄入量平均为48 ± 14 μg/d。血浆硒浓度平均为1.13 ± 0.16 μmol/l。血浆Se与能量校正的Se摄入量（ng Se/kJ/d）显著相关，但Gpx 3活性与Se摄入量（ng Se/kJ/d）和Gpx 3活性与血浆Se均不显著相关。总的来说，这表明受试者处于响应曲线的平台上。从全血中分离的总RNA中定量硒蛋白mRNA，但Gpx 1，硒蛋白H和硒蛋白W（在啮齿动物中均受硒高度调节）的mRNA水平，以及硒蛋白P，Gpx 3和磷脂氢过氧化物谷胱甘肽过氧化物酶也与血浆硒无显著相关性。因此，硒蛋白分子生物标志物，以及传统的生化标志物，是无法进一步区分这些硒充分的主题中的硒状态的差异。分子生物标志物检测硒缺乏症的有效性需要在缺硒人群中进行测试。
Human selenium (Se) requirements are currently based on biochemical markers of Se status. In rats, tissue glutathione peroxidase-1 (Gpx1) mRNA levels can be used effectively to determine Se requirements; blood Gpx1 mRNA levels decrease in Se-deficient rats, so molecular biology-based markers have potential for human nutrition assessment. To study the efficacy of molecular biology markers for assessing Se status in humans, we conducted a longitudinal study on 39 subjects (age 45 ± 11) in Reading, UK. Diet diaries (5 day) and blood were obtained from each subject at 2, 8, 17 and 23 weeks, and plasma Se, glutathione peroxidase (Gpx3) enzyme activity, and selenoprotein mRNA levels were determined. There were no significant longitudinal effects on Se biomarkers. Se intake averaged 48 ± 14 μg/d. Plasma Se concentrations averaged 1·13 ± 0·16 μmol/l. Plasma Se v. energy-corrected Se intake (ng Se/kJ/d) was significantly correlated, but neither Gpx3 activity v. Se intake (ng Se/kJ/d) nor Gpx3 activity v. plasma Se was significantly correlated. Collectively, this indicates that subjects were on the plateaus of the response curves. Selenoprotein mRNAs were quantitated in total RNA isolated from whole blood, but mRNA levels for Gpx1, selenoprotein H, and selenoprotein W (all highly regulated by Se in rodents), as well selenoprotein P, Gpx3, and phospholipid hydroperoxide glutathione peroxidase were also not significantly correlated with plasma Se. Thus selenoprotein molecular biomarkers, as well as traditional biochemical markers, are unable to further distinguish differences in Se status in these Se replete subjects. The efficacy of molecular biomarkers to detect Se deficiency needs to be tested in Se-deficient populations.