Fluorescent Protein-based Biosensors to Visualize Signal Transduction beneath the Plasma Membrane
Fluorescent Protein-based Biosensors to Visualize Signal Transduction beneath the Plasma Membrane
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DOI：
10.2116/analsci.31.267
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发表时间：
2015-04
影响因子：
1.6
通讯作者：
Y. Fujioka;A. Nanbo;S. Nishide;Y. Ohba
中科院分区：
文献类型：
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作者：
Y. Fujioka;A. Nanbo;S. Nishide;Y. Ohba
为了响应细胞外刺激，细胞表现出多种行为，包括增殖、分化、形态变化和迁移。为了更好地理解这种行为，需要对活细胞中信号转导的时空调节进行分析，并且基于荧光蛋白的生物传感器的发展极大地加速了此类研究。目前，通过使用这些生物传感器，可以在活细胞中可视化一系列分子行为，包括脂质代谢、蛋白质激活和离子动力学。我们最近报道，细胞内钙及其相关下游信号通路由小 GTPase Ras 和脂质激酶 phoshoinositide-3-kinase (PI3K) 组成，可利用一组基于荧光蛋白和 Forster 共振能量转移原理的生物传感器，通过内吞作用将甲型流感病毒有效掺入宿主细胞。在这里，我们重点关注我们最近的研究报告中使用的基于荧光蛋白的生物传感器。
In response to extracellular stimuli, cells display a variety of behaviors, including proliferation, differentiation, morphological changes and migration. The analysis of the spatiotemporal regulation of signal transduction in living cells is needed for a better understanding of such behaviors, and such investigations have been greatly accelerated by the development of fluorescent protein-based biosensors. Currently, by using these biosensors a range of molecular actions, including lipid metabolism, protein activation, and ion dynamics, can be visualized in living cells. We recently reported that intracellular calcium, with its relevant downstream signaling pathways consisting of the small GTPase Ras and the lipid kinase phoshoinositide-3-kinase (PI3K), can be exploited in an efficient incorporation of influenza A viruses into host cells via endocytosis using a set of biosensors based on fluorescent proteins and the principle of Forster resonance energy transfer. Here, we focus this review on fluorescent protein-based biosensors that have been utilized in our recent research reports.