Protein Phylogenetic Analysis of Ca(2+)/cation Antiporters and Insights into their Evolution in Plants.
Protein Phylogenetic Analysis of Ca(2+)/cation Antiporters and Insights into their Evolution in Plants.
复制标题
DOI：
10.3389/fpls.2012.00001
复制
发表时间：
2012
影响因子：
5.6
通讯作者：
Pittman JK
中科院分区：
文献类型：
--
作者：
Emery L;Whelan S;Hirschi KD;Pittman JK
阳离子转运在所有生物体中都是一个关键过程，对矿物质营养、离子胁迫耐受性和信号转导至关重要。作为Ca²⁺/阳离子逆向转运蛋白（CaCA）超家族成员的转运蛋白，通过另一种离子（如H⁺或Na⁺）的逆向交换参与Ca²⁺和/或其他阳离子的转运。CaCA超家族先前已被分为五个转运蛋白家族：YRBG、Na⁺/Ca²⁺交换体（NCX）、Na⁺/Ca²⁺，K⁺交换体（NCKX）、H⁺/阳离子交换体（CAX）和阳离子/Ca²⁺交换体（CCX）家族，其中包括特征明确的NCX和CAX转运蛋白。为了研究高等植物和绿色植物谱系中CaCA转运蛋白的进化，从已测序的开花植物、苔藓植物、石松植物以及淡水和海洋藻类的基因组中鉴定出CaCA基因，并与非植物物种的基因进行比较。我们发现了CAX和CCX家族中开花植物基因扩增和多样性增加的证据。与NCX家族相关的基因存在于陆生植物中，尽管它们编码不同的MHX同源物，其转运功能可能已改变。相比之下，陆生植物中缺失的NCX和NCKX基因在许多藻类物种中得以保留，尤其是海洋藻类，这表明这些生物可能具有类似动物的Ca²⁺稳态和信号传导特征。从植物和选定的藻类中鉴定出一组编码含有EF - hand结构域的新型CAX样蛋白的基因，但在其他任何物种中似乎都不存在。由于大多数CaCA蛋白缺乏功能数据，因此无法可靠地预测许多类群或单个蛋白的底物特异性和功能。CaCA基因在生命的所有分支中的丰富性和多样性表明了这类阳离子转运蛋白的重要性，并且许多具有新功能的转运蛋白有待发现。
Cation transport is a critical process in all organisms and is essential for mineral nutrition, ion stress tolerance, and signal transduction. Transporters that are members of the Ca2+/cation antiporter (CaCA) superfamily are involved in the transport of Ca2+ and/or other cations using the counter exchange of another ion such as H+ or Na+. The CaCA superfamily has been previously divided into five transporter families: the YRBG, Na+/Ca2+ exchanger (NCX), Na+/Ca2+, K+ exchanger (NCKX), H+/cation exchanger (CAX), and cation/Ca2+ exchanger (CCX) families, which include the well-characterized NCX and CAX transporters. To examine the evolution of CaCA transporters within higher plants and the green plant lineage, CaCA genes were identified from the genomes of sequenced flowering plants, a bryophyte, lycophyte, and freshwater and marine algae, and compared with those from non-plant species. We found evidence of the expansion and increased diversity of flowering plant genes within the CAX and CCX families. Genes related to the NCX family are present in land plant though they encode distinct MHX homologs which probably have an altered transport function. In contrast, the NCX and NCKX genes which are absent in land plants have been retained in many species of algae, especially the marine algae, indicating that these organisms may share “animal-like” characteristics of Ca2+ homeostasis and signaling. A group of genes encoding novel CAX-like proteins containing an EF-hand domain were identified from plants and selected algae but appeared to be lacking in any other species. Lack of functional data for most of the CaCA proteins make it impossible to reliably predict substrate specificity and function for many of the groups or individual proteins. The abundance and diversity of CaCA genes throughout all branches of life indicates the importance of this class of cation transporter, and that many transporters with novel functions are waiting to be discovered.
登录
查看更多内容
影响因子：
11.6
作者：
Conn, Simon J.;Gilliham, Matthew;Leigh, Roger A.
通讯作者：
Leigh, Roger A.
DOI：
10.1083/jcb.124.3.351
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cunningham KW;Fink GR
通讯作者：
Fink GR
影响因子：
4.8
作者：
Iwamoto, T;Uehara, A;Shigekawa, M
通讯作者：
Shigekawa, M
影响因子：
4.8
作者：
Kamiya, T;Maeshima, M
通讯作者：
Maeshima, M
影响因子：
4.8
作者：
Cagnac, Olivier;Nieves Aranda-Sicilia, Maria;Venema, Kees
通讯作者：
Venema, Kees