Use of the Atomic Force Microscope to Determine the Strength of Bacterial Attachment to Grooved Surface Features
Use of the Atomic Force Microscope to Determine the Strength of Bacterial Attachment to Grooved Surface Features
复制标题
使用原子力显微镜确定细菌附着到凹槽表面特征的强度
DOI：
10.1163/016942410x508019
复制
发表时间：
2010
影响因子：
2.3
通讯作者：
K. Whitehead
中科院分区：
文献类型：
--
作者：
J. Verran;A. Packer;P. Kelly;K. Whitehead
食品接触面的磨损通过磨损和清洗改变了表面粗糙度，并引入了影响微生物保留的地形特征。为了确定特定尺寸特征的影响，制作了具有定义沟槽特征（“光滑”，1.02 μm宽，0.21 μm深沟槽；0.59 μm宽，0.17 μm深沟槽）的表面，这些沟槽特征代表了磨损卫生表面上识别的线性特征的尺寸。利用磁控溅射物理气相沉积（PVD）技术在这些表面上涂覆钛，使表面具有均匀的化学性质。采用原子力显微镜（AFM）研究革兰氏阴性杆状细菌大肠杆菌和革兰氏阳性球菌形细菌严重葡萄球菌对表面的附着强度。AFM尖端在表面上扫描，其中施加的力的增加导致细胞脱离的增加。大肠杆菌细胞很容易通过单独加水从表面去除，而不需要施加侧向力，但去除球菌状细菌的难易程度取决于底层地形。在钛包覆的1.02 μm宽凹槽表面上，S. sciuri保留率最高，而在钛包覆的0.59 μm宽凹槽表面上，S. sciuri保留率最低，这表明sciuri葡萄球菌细胞在与细胞相当的尺寸特征内的保留率最高。这项工作表明，表面特征可以显著影响附着细胞的去除，特别是当地形特征的大小与细菌的大小密切匹配时。
Wear of food contact surfaces through abrasion and cleaning alters surface roughness, and introduces topographical features which affect the retention of microorganisms. In order to identify the effect of features of specific dimensions, surfaces with defined grooved features ('smooth'; 1.02 μm wide, 0.21 μm deep grooves; and 0.59 μm wide, 0.17 μm deep grooves) that are representative of the dimensions of linear features identified on worn hygienic surfaces were fabricated. These surfaces were conformally coated with titanium using the physical vapour deposition (PVD) technique of magnetron sputtering, which provided the surface with a uniform chemistry. Atomic force microscopy (AFM) was used to investigate the strength of attachment of the Gram-negative rod-shaped bacterium Escherichia coli and the Gram-positive coccal-shaped bacterium Staphylococcus sciuri to the surfaces. The AFM tip was scanned across the surface, where an increase in applied force caused an increase in cell detachment. E. coli cells were easily removed from the surfaces by the addition of water alone, without the application of lateral force, but the ease of removal of coccal-shaped bacteria varied depending on the underlying topography. S. sciuri retention was highest on the titanium coated 1.02 μm wide grooved surfaces and least on the titanium coated 0.59 μm wide grooved surfaces, indicating that the Staphylococcus sciuri cells were most strongly held within the features of dimensions comparable to the cells. This work demonstrates that surface features can significantly affect the removal of attached cells, particularly when the size of the topographic features closely matches that of the bacterium.
影响因子：
11.4
作者：
K. Shellenberger;B. Logan
通讯作者：
K. Shellenberger;B. Logan