Effect of chronic treatments with ghrelin on milk secretion in lactating rats.
Effect of chronic treatments with ghrelin on milk secretion in lactating rats.
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DOI：
10.1016/s0006-291x(03)00414-5
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发表时间：
2003-04
影响因子：
3.1
通讯作者：
K. Nakahara;Takahiro Hayashida;M. Nakazato;M. Kojima;H. Hosoda;K. Kangawa;N. Murakami
中科院分区：
文献类型：
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作者：
K. Nakahara;Takahiro Hayashida;M. Nakazato;M. Kojima;H. Hosoda;K. Kangawa;N. Murakami
Ghrelin是一种新的酰化肽，是生长激素促分泌素受体的内源性配体，最初从大鼠和人胃中分离得到。Ghrelin除了具有释放GH的活性外，还在许多生理功能中发挥重要作用，包括食物摄取、胃酸分泌、新生儿发育等，本研究探讨了每天给予Ghrelin对哺乳期大鼠产奶量的影响，并监测了幼鼠的发育情况。每天皮下注射生长激素释放肽到哺乳母鼠8天，从分娩引起显着增加产奶量和窝重增加。在哺乳期第4天，当用生长激素释放肽处理的母鼠和用生理盐水处理的母鼠喂养的窝仔互换时，生长曲线相反。每天注射生长激素释放肽也增加血浆GH水平。北方印迹分析显示，每天注射生长激素释放肽显着增加乳腺酪蛋白mRNA的表达。此外，RT-PCR分析表明，饥饿素受体存在于哺乳大鼠的乳腺。这些结果表明，生长激素释放肽可能发挥重要作用，在泌乳母鼠的牛奶生产。
Ghrelin, a novel acylated peptide and endogenous ligand for growth hormone (GH) secretagogue receptor, was originally isolated from rat and human stomachs. In addition to its GH-releasing activity, ghrelin plays an important role in many physiological functions, including food intake, gastric acid secretion, neonatal development, and so on. In this study, the effect of daily treatment with ghrelin on milk production was investigated in lactating rats and the development of the pups was monitored. Daily subcutaneous injection of ghrelin into nursing dams for 8 days from parturition caused a significant increase in milk yield and litter weight gain. When litters nursed by ghrelin-treated and saline-treated dams were interchanged on day 4 of lactation, the growth curves were reversed. Daily injections of ghrelin also increased plasma GH levels. Northern blot analysis revealed that daily injection of ghrelin significantly increased mammary casein mRNA expression. In addition, RT-PCR analysis showed that a ghrelin receptor was present in the mammary glands of lactating rats. These results suggest that ghrelin may play an important role in milk production in lactating dams.