Killing effect of fish serum and mucus on Gyrodactylus cichlidarum (Platyhelminthes, Monogenea) mediated by complement
Killing effect of fish serum and mucus on Gyrodactylus cichlidarum (Platyhelminthes, Monogenea) mediated by complement
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补体介导的鱼血清和粘液对圆藻（扁形动物、单殖纲）的杀伤作用
DOI：
10.1016/j.aquaculture.2023.739248
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发表时间：
2023-01-23
期刊：
影响因子：
4.5
通讯作者：
Yang, Tingbao
中科院分区：
文献类型：
--
作者：
Chen, Jinsong;Liu, Chang;Yang, Tingbao
陀螺仪主要在鱼类的皮肤和鳍上寄生，代表了养殖鱼类的持续挑战。先前研究的证据表明，补体系统在针对陀螺仪的鱼类中起重要作用。通过一系列用鱼类血清和粘液进行寄生虫杀伤实验，本研究揭示了Oreochromis niloticus补体在杀死Gyrodactylus cichlidarum中的作用。在O. niloticus的血清和粘液中观察到对G. cichlidarum的杀伤作用，在补体抑制热处理和C3抗体中和后，该杀伤作用显着削弱。扫描电子显微镜（SEM）揭示了暴露于O. niloticus血清的G. cichlidarum的Tegument上的各种伤害。在通过Zymosan抑制替代补体途径（ACP）之后，明显减弱了O. niloticus血清对G. cichlidarum的杀伤活性（P <0.01）。在经典补体激活途径（CCP）中抑制（通过将EGTA或L-赖氨酸添加）也导致对杀伤效应的抑制作用较弱。结果表明，O. niloticus补体在杀死G. cichlidarum，主要是通过ACP和CCP的参与所具有必不可少的作用。此外，G. cichlidarum的不自然宿主（ctenopharyngodon idella）的血清中的补体对G. cichlidarum的杀戮作用高于O. niloticus（p <0.01）的杀戮作用，这需要进一步研究其机制。这些发现提供了有关鱼类针对陀螺仪的免疫机制的新证据，并参考了控制陀螺氏病的研究。
Gyrodactylids mainly parasitizing on the skin and fins of fishes, represent an ongoing challenge of farmed fishes. Evidences from previous studies suggested that complement system play important role in fish against gyrodactylids. Through a series of parasite killing experiments with fish sera and mucus, the present study revealed the role of Oreochromis niloticus complement in killing Gyrodactylus cichlidarum. The killing effect on G. cichlidarum was observed in serum and mucus of O. niloticus, which was markedly weakened after the complement inhibition by heat treatment and C3 antibody neutralization. Scanning electron microscopy (SEM) revealed various injuries on the tegument of G. cichlidarum exposed to O. niloticus serum. After inhibiting the alternative complement pathway (ACP) by zymosan, the killing activity of O. niloticus serum to G. cichlidarum was significantly attenuated (P < 0.01). The inhibition (by adding EGTA or L-lysine) to the classical complement activation pathway (CCP) also resulted a weak suppression on the killing effect. The results demonstrated the indispensable role of O. niloticus complement in killing G. cichlidarum, mainly via the ACP and with the involvement of CCP as well. In addition, complement in serum of an unnatural host (Ctenopharyngodon idella) of G. cichlidarum showed highly significantly weaker killing effect to G. cichlidarum than that of O. niloticus (P < 0.01), which needs further study for its mechanism. These findings provided new evidences about the immune mechanism of fish against gyrodactylids, and are reference for the study on the control of gyrodactyliasis.