Age-related changes in fatty acids from different adipose depots in rat and their association with adiposity and insulin
Age-related changes in fatty acids from different adipose depots in rat and their association with adiposity and insulin
复制标题
DOI：
10.1016/j.nut.2008.03.022
复制
发表时间：
2008-10-01
期刊：
影响因子：
4.4
通讯作者：
Garaulet, Marta
中科院分区：
文献类型：
--
作者：
Perez de Heredia, Fatima;Larque, Elvira;Garaulet, Marta
目的：通过比较不同脂肪部位大鼠血清和脂肪组织中脂肪酸(FAs)的年龄相关性变化，分析其与肥胖和胰岛素功能的关系。方法：雌性断奶大鼠分别饲喂高脂饲料至6、14、20mo龄(n=12，n=6，n=10)。测量体重、肥胖度、血清胰岛素、血糖和稳态模型评估指数。结果：体重和肥胖度随年龄增长而增加，内脏血库随年龄增长而肥大，皮下血库随年龄增长而增加，皮下血库随年龄增长而增加。最初，肠系膜组织表现出更高的饱和和反式FA含量，而棕色组织具有更高的多不饱和脂肪酸(PUFA)比例。老化导致脂肪组织的饱和度较低，削弱了不同储存库之间的早期差异。血清和脂肪组织中的omega-6多不饱和脂肪酸随着年龄的增长而升高，主要是由于C18：2 omega-6，而长链PUFAs，C20：5 omega-3和C22：6 omega-3有下降的趋势。肥胖与单不饱和脂肪酸呈正相关，与多不饱和脂肪酸呈负相关；胰岛素相关变量与血清w-6多不饱和脂肪酸呈负相关，与血清单不饱和脂肪酸和皮下脂肪含量呈正相关。在喂食高脂饮食的大鼠中，衰老导致较低的饱和度，血清和脂肪组织中心脏代谢风险因子omega-6/omega-3的比值增加。(C)2008 Elsevier Inc.保留所有权利。
Objective: We studied age-related changes in fatty acids (FAs) from serum and adipose tissue in rats by comparing different adipose regions and analyzed their relations to adiposity and insulin function.Methods: Female weaned rats were fed on a high-fat diet until 6, 14, and 20 mo of age (n = 12, n = 6, n 10, respectively). Body weight, adiposity, serum insulin, serum glucose, and homeostatic model assessment index were measured. FA compositions from serum and interscapular brown, periovarian, mesenteric, and subcutaneous tissues were determined by gas chromatography.Results: Body weight and adiposity increased with age; visceral depots grew by hypertrophy, whereas subcutaneous depots grew by hyperplasia and in a higher ratio. Initially, the mesenteric tissue showed greater saturated and trans-FA contents, whereas brown tissue had higher polyunsaturated FA (PUFA) proportions. Aging resulted in a lower saturation degree in adipose tissue, attenuating earlier differences among depots. There was an elevation in omega-6 PUFAs with age, mainly because of C18:2 omega-6, whereas omega-3 long-chain PUFAs, C20:5 omega-3 and C22:6 omega-3, tended to decrease in serum and adipose tissue. Adiposity was associated positively with monounsaturated FAs and inversely with PUFAs; insulin-related variables correlated negatively with serum w-6 PUFA but positively with serum monounsaturated FAs and subcutaneous depot trans-FAs.Conclusion: The mesenteric tissue showed the least favorable FA profile compared with the other depots, but differences among adipose regions diminished with age. In rats fed a high-fat diet, aging resulted in a lower saturation degree, with increased values in the cardiometabolic risk factor omega-6/omega-3 ratio in serum and adipose tissue. (C) 2008 Elsevier Inc. All rights reserved.