A simple and reversible glass-glass bonding method to construct a microfluidic device and its application for cell recovery
A simple and reversible glass-glass bonding method to construct a microfluidic device and its application for cell recovery
复制标题
DOI：
10.1039/d1lc00058f
复制
发表时间：
2021-04-27
期刊：
影响因子：
6.1
通讯作者：
Tanaka, Yo
中科院分区：
文献类型：
--
作者：
Funano, Shun-ichi;Ota, Nobutoshi;Tanaka, Yo
与聚合物微流控器件相比，玻璃微流控器件由于其刚性、光学透明性、热稳定性和化学/生物惰性而在各种芯片实验室应用中具有优势。然而，构造玻璃微流体装置的结合过程通常涉及处理，如超过400摄氏度的高温、氧等离子体或食人鱼溶液。这些过程需要特殊的技能、设备或苛刻的化学品，并破坏微通道中的分子或细胞。在这里，我们提出了一种简单的方法，玻璃-玻璃键合，很容易形成微通道。该方法由两个步骤组成：将水滴放置在用中性洗涤剂清洁的玻璃基板上，然后在室温下通过结合夹将盖板玻璃板固定在玻璃基板上几个小时。表面分析表明，玻璃表面清洗的中性洗涤剂具有较高的SiOH比SiO比由其他清洗步骤制备的玻璃表面。因此，由于SiOH的密度较高，所建议的方法可以通过脱水缩合实现更强的玻璃-玻璃结合。在6小时的键合内，压力耐受性达到超过600 kPa，这对于实际的微流体应用是足够的。此外，通过利用这种结合方法的可逆性，证明了在微通道中培养细胞后的细胞回收。这种新的键合方法可以显著提高玻璃微流控器件的生产率和可用性，并扩展玻璃微流控应用的可能性。
Compared with polymer microfluidic devices, glass microfluidic devices have advantages for diverse lab-on-a-chip applications due to their rigidity, optical transparency, thermal stability, and chemical/biological inertness. However, the bonding process to construct glass microfluidic devices usually involves treatment(s) like high temperature over 400 degrees C, oxygen plasma or piranha solution. Such processes require special skill, apparatus or harsh chemicals, and destroy molecules or cells in microchannels. Here, we present a simple method for glass-glass bonding to easily form microchannels. This method consists of two steps: placing water droplets on a glass substrate cleaned by neutral detergent, followed by fixing a cover glass plate on the glass substrate by binding clips for a few hours at room temperature. Surface analyses showed that the glass surface cleaned by neutral detergent had a higher ratio of SiOH over SiO than glass surfaces prepared by other cleaning steps. Thus, the suggested method could achieve stronger glass-glass bonding via dehydration condensation due to the higher density of SiOH. The pressure endurance reached over 600 kPa within 6 h of bonding, which is sufficient for practical microfluidic applications. Moreover, by exploiting the reversibility of this bonding method, cell recoveries after cultivating cells in a microchannel were demonstrated. This new bonding method can significantly improve both the productivity and the usability of glass microfluidic devices and extend the possibility of glass microfluidic applications in future.