Transcriptional regulation of the leukocyte adherence protein beta subunit during human myeloid cell differentiation.
Transcriptional regulation of the leukocyte adherence protein beta subunit during human myeloid cell differentiation.
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DOI：
10.1016/s0021-9258(18)68323-1
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发表时间：
1988-09
期刊：
影响因子：
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通讯作者：
Dennis D. HicksteinSOT;M. Hickey;S. Collins
中科院分区：
文献类型：
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作者：
Dennis D. HicksteinSOT;M. Hickey;S. Collins
涉及人白细胞的粘附反应由一个糖蛋白表面抗原家族介导，该家族由三种不同的α亚基（称为α L、α M和α X）组成，每种α亚基与α 1 β 1异二聚体结构中的单个β亚基相关。我们克隆了共同β亚基的cDNA，并研究了β亚基mRNA在HL-60早幼粒细胞白血病细胞和人粒细胞中的表达。白细胞粘附受体β亚基mRNA转录本在HL-60成髓细胞和早幼粒细胞中以低水平存在，并且随着化学诱导分化为更成熟的粒细胞（使用视黄酸和二甲基甲酰胺）或单核细胞/巨噬细胞（使用佛波醇）而增加10倍或更多肉豆蔻酸醋酸酯）。在正常人外周血粒细胞和慢性粒细胞白血病患者的粒细胞中，β亚基mRNA的表达水平也增加。核径流分析表明，在维甲酸分化的HL-60细胞中，β亚基mRNA的稳态水平的增加是次要的增强β亚基基因转录。我们的结论是，在更成熟的粒细胞相比，未成熟的髓系前体细胞的人白细胞介导细胞粘附的受体的β亚基的mRNA水平增加，这种增强的mRNA表达是转录调节。
Adherence reactions involving human leukocytes are mediated by a family of glycoprotein surface antigens composed of three different alpha subunits designated alpha L, alpha M, and alpha X, each of which is associated with a single beta subunit in an alpha 1 beta 1 heterodimer structure. We cloned the cDNA for the common beta subunit and investigated beta subunit mRNA expression in HL-60 promyelocytic leukemia cells and human granulocytic cells. Leukocyte adherence receptor beta subunit mRNA transcripts were present in low levels in HL-60 myeloblasts and promyelocytes and increased 10-fold or greater with chemically induced differentiation to more mature granulocytes (using retinoic acid and dimethylformamide) or monocyte/macrophages (using phorbol myristate acetate). Levels of beta subunit mRNA expression were also increased both in normal human peripheral blood granulocytes and in granulocytes from patients with chronic myelogenous leukemia. Nuclear run-off assays indicated that the increased steady state level of the beta subunit mRNA in retinoic acid-differentiated HL-60 cells was secondary to enhanced beta subunit gene transcription. We conclude that mRNA levels for the beta subunit of the receptor on human leukocytes that mediates cellular adherence are increased in more mature granulocytic cells compared to immature myeloid precursors and that this enhanced mRNA expression is transcriptionally regulated.