Brown adipose tissue mapping in rats with combined intermolecular double-quantum coherence and Dixon water-fat MRI
Brown adipose tissue mapping in rats with combined intermolecular double-quantum coherence and Dixon water-fat MRI
复制标题
结合分子间双量子相干和 Dixon 水脂肪 MRI 绘制大鼠棕色脂肪组织图
DOI：
10.1002/nbm.3000
复制
发表时间：
2013-12-01
影响因子：
2.9
通讯作者：
Chen, Zhong
中科院分区：
文献类型：
--
作者：
Bao, Jianfeng;Cui, Xiaohong;Chen, Zhong
棕色脂肪组织（BAT）是肥胖研究中一个有前途的治疗靶点。最近，MRI 已被提议用于 BAT 的绘图。然而，由于空间分辨率的限制，类似于现有用于BAT检测的正电子发射断层扫描和计算机断层扫描技术，当BAT细胞与其他细胞混合时，无法区分BAT细胞。在这项工作中，提出了一种新的MRI方法，结合了分子间双量子相干性和化学位移编码Dixon方法。其对比度取决于细胞尺度的水脂肪比，该比值小于成像体素尺寸。通过计算机模拟和模型以及7 T下大鼠BAT的初步成像证明了该MRI方法的可行性。计算机模拟和实验结果与理论预测一致。该方法提供了一种新颖的对比机制，可以专门绘制BAT分布图。特别是，在老年大鼠中检测到了 BAT 细胞和白色脂肪组织细胞的混合物，这是其他非侵入性方法无法检测到的。该方法可能适用于 BAT 相关研究的广泛用途，包括 BAT 的形成和变异。版权所有 (c) 2013 John Wiley & Sons, Ltd.
Brown adipose tissue (BAT) is a promising therapeutic target in obesity studies. Recently, MRI has been proposed for the mapping of BAT. However, because of the limitation of spatial resolution, similar to the existing positron emission tomography and computed tomography techniques for BAT detection, it fails to distinguish BAT cells when they are mixed with other cells. In this work, a new MRI method is proposed, combining intermolecular double-quantum coherence and the chemical shift-encoded Dixon method. Its contrast depends on the water to fat ratio at the cellular scale, which is smaller than the imaging voxel size. The feasibility of this MRI method was shown with computer simulations and phantoms, and preliminary imaging of BAT of rats at 7 T. Both computer simulations and experimental results are consistent with theoretical predictions. The method provides a novel contrast mechanism and can map BAT distribution exclusively. In particular, a mixture of BAT cells and white adipose tissue cells was detected in an older rat, which was undetectable by other noninvasive methods. This method may be applicable to a wide range of uses in BAT-related studies, including the formation and variation of BAT. Copyright (c) 2013 John Wiley & Sons, Ltd.