Genetic polymorphisms of the renin-angiotensin system and obesity-related metabolic changes in response to low-energy diets in obese women
Genetic polymorphisms of the renin-angiotensin system and obesity-related metabolic changes in response to low-energy diets in obese women
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DOI：
10.1016/j.nut.2009.10.012
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发表时间：
2011-01-01
期刊：
影响因子：
4.4
通讯作者：
Sakane, Naoki
中科院分区：
文献类型：
--
作者：
Hamada, Taku;Kotani, Kazuhiko;Sakane, Naoki
目的：肾素-血管紧张素系统基因多态性与心血管及代谢性疾病有关。本研究的目的是探讨血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性和血管紧张素II 2型受体（AT（2）R）基因3123 C/A多态性是否会影响血压和其他肥胖相关的代谢变化，以响应使用代餐奶昔减肥的低能量饮食。对32名肥胖妇女（年龄49.9 ± 8.4 [SD] y; BMI 28.4 ± 3.3 kg/m2）进行了2个月的干预，干预前和干预后测量了临床、代谢和生化特征，限制为1200 kcal/d（5021 kJ/d）。用嵌入剂介导的FRET探针检测系统检测多态性。虽然体重减轻和营养摄入水平在基因型之间没有差异，但体重减轻后，ACE缺失/缺失（D/D）基因型的体脂减少量显著低于插入/插入（I/I）+I/D基因型（-2.25 +/- 1.40%对比-0.80 +/-1.57%，P < 0.05）。AT（2）RA/A组收缩压水平的改善明显较少（-7.23 +/- 8.50 vs 2.50 +/- 12.6 mmHg，P < 0.05），低密度脂蛋白-胆固醇（-0.36 +/- 0.29 vs-0.09 +/- 0.25 mmol/L，P < 0.05），碳水化合物（-54.4 +/- 27.2对比-31.8 +/- 16.3 mg/min，P < 0.05）和脂肪氧化（8.31 +/- 11.86 vs 0.05 +/- 9.99 mg/min，P < 0.05）。目前的研究结果表明，ACE基因的纯合形式可能会阻碍体脂的改善，AT（2）R基因的纯合形式可能会改善收缩压和某些肥胖症。相关代谢参数，通过饮食干预肥胖妇女的困难。(C)2011 Elsevier Inc. All rights reserved.
Objective: Genetic polymorphisms of the renin-angiotensin system have been implicated in cardiovascular and metabolic diseases. The purpose of this study was to investigate whether the insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) gene and 3123C/A polymorphism of the angiotensin II type 2 receptor (AT(2)R) gene affect blood pressure and other obesity-related metabolic changes in response to low-energy diets using meal replacement shakes for weight loss.Methods: Clinical, metabolic, and biochemical profiles were measured before and after a 2-mo intervention in 32 obese women (age 49.9 +/- 8.4 [SD] y; BMI 28.4 +/- 3.3 kg/m(2)) restricted to 1200 kcal/d (5021 kJ/d). The polymorphisms were determined with an intercalater-mediated FRET probe assay system.Results: Although weight loss and nutrient intake levels did not differ among the genotypes, the reduction in body fat after weight loss was significantly less in the ACE deletion/deletion (D/D) genotype than insertion/insertion (I/I) plus I/D genotype (-2.25 +/- 1.40% versus -0.80 +/- 1.57%, P < 0.05). The AT(2)R A/A group had significantly less improved levels of systolic blood pressure (-7.23 +/- 8.50 versus 2.50 +/- 12.6 mmHg, P < 0.05), low-density lipoprotein-cholesterol (-0.36 +/- 0.29 versus -0.09 +/- 0.25 mmol/L, P < 0.05), carbohydrate (-54.4 +/- 27.2 versus -31.8 +/- 16.3 mg/min, P < 0.05) and fat oxidation (8.31 +/- 11.86 versus 0.05 +/- 9.99 mg/min, P < 0.05) than the C/C plus C/A genotypes.Conclusion: The present findings suggest that the homozygous form of the ACE gene may hinder the improvement of body fat and that the homozygous form of the AT(2)R gene may make improving systolic blood pressure and some obesity-related metabolic parameters through a dietary intervention difficult among obese women. (C) 2011 Elsevier Inc. All rights reserved.