Monitoring of urea and potassium by reverse iontophoresis in vitro.
Monitoring of urea and potassium by reverse iontophoresis in vitro.
复制标题
通过体外反向离子电渗疗法监测尿素和钾。
DOI：
10.1007/s11095-007-9237-0
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发表时间：
2007
影响因子：
3.7
通讯作者：
Préat,Véronique
中科院分区：
文献类型：
--
作者：
Wascotte,Valentine;Delgado-Charro,MBegoña;Rozet,Eric;Wallemacq,Pierre;Hubert,Philippe;Guy,RichardH;Préat,Véronique
PurposeReverse iontophoresis is an alternative to blood sampling for the monitoring of endogenous molecules. Here, the potential of the technique to measure urea and potassium levels non-invasively, and to track their concentrations during hemodialysis, has been examined.Materials and MethodsIn vitroexperiments were performed to test (a) a series of subdermal urea and potassium concentrations typical of the pathophysiologic range, and (b) a decreasing profile of urea and potassium subdermal concentrations to mimic those which are observed during hemodialysis.Results(a) After 60–120 min of iontophoresis, linear relationships (p< 0.05) were established between both urea and potassium fluxes and their respective subdermal concentrations. The determination coefficients were above 0.9 after 1 h of current passage using sodium as an internal standard. (b) Reverse iontophoretic fluxes of urea and K+closely paralleled the decay of the respective concentrations in the subdermal compartment, as would occur during a hemodialysis session.ConclusionsThesein vitroexperiments demonstrate that urea and potassium can be quantitatively and proportionately extracted by reverse iontophoresis, even when the subdermal concentrations of the analytes are varying with time. These results suggest the non-invasive monitoring of urea and potassium to diagnose renal failure and during hemodialysis is feasible, and thatin vivomeasurements are warranted.
