Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin
Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin
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DOI：
10.1016/j.bbrc.2007.05.188
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发表时间：
2007-08-03
影响因子：
3.1
通讯作者：
Tagaya, Mitsuo
中科院分区：
文献类型：
--
作者：
Arasaki, Kohei;Uemura, Takefumi;Tagaya, Mitsuo
ZW 10通过与动力蛋白附件复合物动力肌动蛋白的一个亚基dynitin相互作用，参与有丝分裂期间纺锤体检查点的终止。我们以前表明，ZW 10是通过RINT-1附着在内质网在间期HeLa细胞和参与内质网和高尔基体之间的膜运输。虽然最近的研究表明，ZW 10定位于COS 7细胞中的高尔基体，但调节ZW 10定位的机制仍然未知。在这项研究中，我们发现ZW 10的高尔基体定位和dynactin的中心体积累之间的相关性。ZW 10与dynactin的相关量在ZW 10存在于高尔基体中的细胞中大于ZW 10不在高尔基体中的细胞。ZW 10的核周高尔基体区域的靶向被发现依赖于dynactin的核周积累，这表明dynactin调节ZW 10的定位。(c)2007爱思唯尔公司All rights reserved.
ZW10 participates in the termination of the spindle checkpoint during mitosis by interacting with dynamitin, a subunit of the dynein accessory complex dynactin. We previously showed that ZW10 is attached to the endoplasmic reticulum through RINT-1 in interphase HeLa cells and involved in membrane transport between the endoplasmic reticulum and Golgi. Although a recent study demonstrated that ZW10 is localized in the Golgi in COS7 cells, the mechanism that regulates ZW10 localization remains unknown. In this study we showed a correlation between the Golgi localization of ZW10 and the centrosomal accumulation of dynactin. The amounts of ZW10 associated with dynactin were larger in cells where ZW10 was present in the Golgi than those where ZW10 was not in the Golgi. The targeting of ZW10 to the perinuclear Golgi region was found to depend on the perinuclear accumulation of dynactin, suggesting that dynactin regulates ZW10 localization. (c) 2007 Elsevier Inc. All rights reserved.