Effect of UVA fluence rate on indicators of oxidative stress in human dermal fibroblasts.
Effect of UVA fluence rate on indicators of oxidative stress in human dermal fibroblasts.
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DOI：
10.7150/ijbs.4.63
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发表时间：
2008-02-19
影响因子：
9.2
通讯作者：
Vanderhill L
中科院分区：
文献类型：
--
作者：
Hoerter JD;Ward CS;Bale KD;Gizachew AN;Graham R;Reynolds J;Ward ME;Choi C;Kagabo JL;Sauer M;Kuipers T;Hotchkiss T;Banner N;Chellson RA;Ohaeri T;Gant L;Vanderhill L
在一天的过程中，人类皮肤暴露于在UVA（290-315 nm）和UVB（315-400 nm）光谱内的通量率波动的太阳UV辐射。影响到达皮肤细胞的注量率的变量包括UVA和UVB穿透能力的差异、防晒霜的存在或不存在、大气条件以及暴露发生的季节和地理位置。我们的研究确定了在太阳模拟（SSR）和鞣革床辐射（TBR）的UVA能量密度率的影响对四个指标的氧化应激-蛋白质氧化，谷胱甘肽，血红素加氧酶-1，和活性氧物种-在人皮肤成纤维细胞后，接收等效的UVA和UVB剂量。我们的研究结果表明，TBR中较高的UVA通量率增加了所有四个氧化应激指标的水平。在连续暴露时，细胞首先暴露于SSR，SSR中较低的UVA能流率诱导保护性反应，该保护性反应在第二次暴露于较高的UVA能流率后防止氧化应激。我们的研究强调了UVA注量率在确定人体皮肤细胞如何对给定剂量的UVA和UVB辐射做出反应方面的重要作用。
During the course of a day human skin is exposed to solar UV radiation that fluctuates in fluence rate within the UVA (290-315 nm) and UVB (315-400 nm) spectrum. Variables affecting the fluence rate reaching skin cells include differences in UVA and UVB penetrating ability, presence or absence of sunscreens, atmospheric conditions, and season and geographical location where the exposure occurs. Our study determined the effect of UVA fluence rate in solar-simulated (SSR) and tanning-bed radiation (TBR) on four indicators of oxidative stress---protein oxidation, glutathione, heme oxygenase-1, and reactive oxygen species--in human dermal fibroblasts after receiving equivalent UVA and UVB doses. Our results show that the higher UVA fluence rate in TBR increases the level of all four indicators of oxidative stress. In sequential exposures when cells are exposed first to SSR, the lower UVA fluence rate in SSR induces a protective response that protects against oxidative stress following a second exposure to a higher UVA fluence rate. Our studies underscore the important role of UVA fluence rate in determining how human skin cells respond to a given dose of radiation containing both UVA and UVB radiation.