Ultra-performance liquid chromatography coupled with graphene/polyaniline nanocomposite modified electrode for the determination of sulfonamide residues.
Ultra-performance liquid chromatography coupled with graphene/polyaniline nanocomposite modified electrode for the determination of sulfonamide residues.
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DOI：
10.1016/j.talanta.2014.02.004
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发表时间：
2014-06
期刊：
影响因子：
6.1
通讯作者：
Nupattaranee Thammasoontaree;P. Rattanarat;N. Ruecha;W. Siangproh;N. Rodthongkum;O. Chailapakul
中科院分区：
文献类型：
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作者：
Nupattaranee Thammasoontaree;P. Rattanarat;N. Ruecha;W. Siangproh;N. Rodthongkum;O. Chailapakul
建立了石墨烯/聚苯胺（G/PANI）修饰的丝网印刷碳电极耦合超高效液相色谱（UPLC）分离和灵敏测定虾中8种磺胺类物质的方法。选择电喷涂修饰电极是因为电喷涂可以使G/聚苯胺纳米复合材料在电极表面分散良好。在电化学检测之前，采用反相UPLC (C4)，流动相为70:25:5 （v/v/v）的磷酸氢钾（pH 3）：乙腈：乙醇，在7 min内将8种SAs完全分离。对于安培检测，流体动力伏安法获得的检测电位为+1.4 V。在最优条件下，8种sa在0.01 ~ 10µg mL - 1和1.162 ~ 6.127 ng mL - 1范围内具有较宽的线性关系和较低的检出限。与掺硼金刚石（BDD）电极相比，G/聚苯胺修饰的丝网印刷碳电极具有更高的灵敏度和更低的运行成本。采用固相萃取法在UPLC分离前对样品进行清理和预浓缩，测定虾类样品中的SAs。为了验证所建立的方法，在UPLC-UV系统中完成了高度定量的一致性。因此，该系统可能是一种快速、廉价、灵敏地测定对虾中砷含量的替代方法。
An ultra-performance liquid chromatography (UPLC) coupled with graphene/polyaniline (G/PANI)-modified screen-printed carbon electrode was developed for separation and sensitive determination of eight sulfonamides (SAs) in shrimp. Electrospraying was selected for electrode modification because it can generate the well dispersion of G/PANI nanocomposites on the electrode surface. Prior to electrochemical detection, eight SAs were completely separated within 7 min by using reversed phase UPLC (C4) with mobile phase containing 70:25:5 (v/v/v) of potassium hydrogen phosphate (pH 3):acetonitrile:ethanol. For amperometric detection, the detection potential acquired from hydrodynamic voltammetry was found to be +1.4 V. Under optimal conditions, a wide linearity and low limit of detection were obtained for eight SAs in the range of 0.01–10 µg mL−1and 1.162–6.127 ng mL−1, respectively. Compared to boron-doped diamond (BDD) electrode, a G/PANI-modified screen-printed carbon electrode offered higher sensitivity with lower operating cost. To determine SAs in shrimp samples, solid-phase extraction was used to clean up and preconcentrate the samples prior to UPLC separation. To validate this developed method, a highly quantitative agreement was accomplished with UPLC–UV system. Thus, this proposed system might be an alternative approach for rapid, inexpensive, and sensitive determination of SAs in shrimps.