DISSOCIATION BY COLCHICINE OF PHAGOCYTOSIS FROM INCREASED OXYGEN CONSUMPTION IN HUMAN LEUKOCYTES
DISSOCIATION BY COLCHICINE OF PHAGOCYTOSIS FROM INCREASED OXYGEN CONSUMPTION IN HUMAN LEUKOCYTES
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DOI：
10.1172/jci105579
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发表时间：
1967-01-01
影响因子：
15.9
通讯作者：
BODEL, PT
中科院分区：
文献类型：
--
作者：
MALAWISTA, SE;BODEL, PT
将秋水仙碱对活链球菌或灭活葡萄球菌吞噬作用期间人类白细胞吸收氧气的影响与在敏感细菌系统中测量的秋水仙碱对吞噬作用本身的影响进行比较。在用浓度低至2.5×10-6摩尔/L(1μg/ml)的秋水仙碱孵育的白细胞中，通常伴随吞噬作用的耗氧量的增加持续减少，并且这种抑制是剂量依赖性的。然而，没有证据表明秋水仙碱浓度高达2.5×10-4摩尔/升(100微克/毫升)时吞噬作用降低。此外，在 20、40 和 60 分钟时进行测量，吞噬率与有秋水仙碱和无秋水仙碱的情况相当。在快速干燥的孵育白细胞制剂中发现了耗氧量和吞噬作用之间解离的线索。对照和秋水仙碱处理的粒细胞中都存在摄入的细菌。此外，对照细胞显示颗粒（溶酶体颗粒）的正常损失和明显的细胞质空泡（消化空泡）。然而，秋水仙碱处理的细胞表现出较少的脱颗粒和空泡化。吞噬作用后颗粒相关酸性磷酸酶活性的测量支持了秋水仙碱处理的白细胞中较少脱颗粒的形态学观察结果。秋水仙碱处理的细胞中对吞噬作用的弱代谢和形态学反应可能对于秋水仙碱在急性痛风性关节炎中的抗炎作用很重要。秋水仙碱还可能在定义代谢刺激细胞的结构功能依赖性方面有更广泛的用途。
The effect of colchicine on the uptake of oxygen by human leukocytes during phagocytosis of live streptococci or of killed staphylococci was compared with the effect of colchicine on phagocytosis per se, measured in a sensitive bacterial system. The increase in oxygen consumption that normally accompanies phagocytosis was consistently diminished in leukocytes incubated with colchicine in concentrations as low as 2.5 x 10-6 mole per L (1 [mu]g per ml), and this inhibition was dosage dependent. Yet there was no evidence of decreased phagocytosis with concentrations of colchicine as high as 2.5 x 10-4 mole per L (100 [mu]g per ml). Furthermore, with measurements at 20, 40, and 60 min., the rate of phagocytosis was comparable with and without colchicine. A clue to the dissociation between oxygen consumption and phagocytosis was found in rapidly dried preparations of the incubated leukocytes. Ingested bacteria were present in both control and colchicine-treated granulocytes. In addition, control cells showed normal loss of granules (lysosomal particles) and prominent cytoplasmic vacuoles (digestive vacuoles). Colchicine-treated cells, however, showed less such degranulation and vacuolization. Measurements of granule-associated acid phosphatase activity after phagocytosis support the morphologic observations of less degranulation in colchicine-treated leukocytes. The muted metabolic and morphologic response to phagocytosis in colchicine-treated cells may be important for the anti-inflammatory effect of colchicine in acure gouty arthritis. Colchicine may also find wider use in defining structure-function de-pendencies in metabolically stimulated cells.