CELL-TYPE-SPECIFIC CDNA PROBES AND THE MURINE-I REGION - THE LOCALIZATION AND ORIENTATION OF A-ALPHA-D
CELL-TYPE-SPECIFIC CDNA PROBES AND THE MURINE-I REGION - THE LOCALIZATION AND ORIENTATION OF A-ALPHA-D
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DOI：
10.1073/pnas.81.7.2194
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
HOOD, L
中科院分区：
文献类型：
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作者：
DAVIS, MM;COHEN, DI;HOOD, L
高度富集 B 细胞特异性 (B*-T) 和 T 抑制细胞特异性的标记 c[互补]DNA 探针。**图形**。序列用于筛选一组跨越鼠免疫反应 (I) 区域 230 KB 的基因组粘粒克隆。使用 B 细胞衍生的探针，可检测到 4 个 I 区基因（A.beta.、E.beta.、E.beta.2 和 E.alpha.）以及一个额外的（第 5 个）杂交区域。由假定的 I-J 阳性抑制细胞杂交瘤制备的 T 细胞探针在平行实验中呈阴性。对应于用 B 细胞 cDNA 探针观察到的新杂交区域的基因组片段在 RNA 印迹分析中鉴定出一种离散的 mRNA 种类，其表达模式与 E.alpha 惊人相似。多种淋巴肿瘤系中的 mRNA。该片段还用于从 B 细胞特异性序列高度富集（20-40 倍）的文库中分离 cDNA 克隆（选定的 cDNA 克隆）。对这些 cDNA 克隆之一的 DNA 序列分析表明该基因编码 A.alpha。 d 单倍型的分子。**图形**。 Ia复合体中II类基因的顺序如下：着丝粒-A.beta.-A.alpha.-E.beta.-E.beta.2-E.alpha。
Labeled c[complementary]DNA probes highly enriched for B-cell-specific (B*-T) and T-suppressor-cell-specific .**GRAPHIC**. sequences were used to screen a set of genomic cosmid clones spanning 230 kilobases of the murine immune response (I) region. With the B-cell derived probe, 4 I-region genes were detectable (A.beta., E.beta., E.beta.2 and E.alpha.), as well as an additional (5th) region of hybridization. The T-cell probe, prepared from a putative I-J positive suppressor cell hybridoma, was negative in a parallel experiment. A genomic fragment corresponding to the new region of hybridization seen with the B-cell cDNA probe identified a discrete mRNA species in RNA blotting analysis that had a pattern of expression strikingly similar to E.alpha. mRNA in a variety of lymphoid tumor lines. This fragment was also used to isolate cDNA clones from a library highly enriched (20-40 times) for B-cell-specific sequences (selected cDNA cloning). DNA sequence analysis of one of these cDNA clones indicates that this gene encodes the A.alpha. molecule of the d haplotype .**GRAPHIC**. The order of the class II genes in the Ia complex is as follows: centromere-A.beta.-A.alpha.-E.beta.-E.beta.2-E.alpha.