Chromatographic deuterium isotope effects of derivatized N-glycans and N-glycopeptides in a zwitterionic type of hydrophilic interaction chromatography.
Chromatographic deuterium isotope effects of derivatized N-glycans and N-glycopeptides in a zwitterionic type of hydrophilic interaction chromatography.
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DOI：
10.1002/jssc.200700659
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发表时间：
2008-05
影响因子：
3.1
通讯作者：
Yasuhiro Takegawa;M. Hato;Kisaburo Deguchi;H. Nakagawa;S. Nishimura
中科院分区：
文献类型：
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作者：
Yasuhiro Takegawa;M. Hato;Kisaburo Deguchi;H. Nakagawa;S. Nishimura
HPLC/MS 现在是分析聚糖和糖肽的重要方法。使用 ICAT 的技术也因其基于 MS 信号强度的比较/定量分析而变得流行。然而，最常用的 RP HPLC 会产生氘标记和非标记肽的“双峰”，众所周知，这会引起色谱氘同位素效应。对于精确比较 MS 信号强度来说，这种双峰是不受欢迎的。该报告表明，在两性离子型亲水相互作用色谱中，对于用氘标记的2-氨基吡啶衍生的N-聚糖和用氘标记的琥珀酸酐衍生的N-糖肽，色谱氘同位素效应小到可以忽略不计。
HPLC/MS is now an essential method for the analysis of glycans and glycopeptides. A technique using ICATs is also becoming popular for their comparative/quantitative analysis based on MS signal intensities. However, the RP HPLC most often used causes "doublet" peaks of deuterium-labeled and nonlabeled peptides, which are well known for causing the chromatographic deuterium isotope effect. Such doublet peaks are undesirable for precise comparison of MS signal intensities. This report shows that, in the zwitterionic type of hydrophilic interaction chromatography, the chromatographic deuterium isotope effect is negligibly small for N-glycans derivatized with deuterium-labeled 2-aminopyridine and N-glycopeptides derivatized with deuterium-labeled succinic anhydride.