Identification of CNS Neurons Innervating the Levator Ani and Ventral Bulbospongiosus Muscles in Male Rats
Identification of CNS Neurons Innervating the Levator Ani and Ventral Bulbospongiosus Muscles in Male Rats
复制标题
DOI：
10.1111/jsm.12418
复制
发表时间：
2014-03-01
影响因子：
3.5
通讯作者：
Marson, Lesley
中科院分区：
文献类型：
--
作者：
Dobberfuhl, Amy D.;Oti, Takumi;Marson, Lesley
盆腔横纹肌在调节勃起和射精中起着重要的作用，这些肌肉一起组成了一个紧密协调的神经肌肉系统，是雄激素敏感的和两性二态的。目的探讨成年和青春期前雄性大鼠提肛肌（LA）和球海绵肌（BS）的脊髓和脑神经元调控机制。方法对大鼠进行麻醉，将经突触逆行示踪剂伪狂犬病毒（PRV）注射到30日龄大鼠的LA肌或BS肌腹侧。3-5天后处死大鼠，用免疫组织化学方法鉴定脊髓和脑内prv标记的神经元。检测腰脊髓神经元胃泌素释放肽（GRP）的存在。prv标记的神经元在脊髓和脑中的位置和数量以及GRP在腰椎的共定位。结果脊髓内侧背侧运动神经元感染后，sprv标记的脊髓中间神经元分布于脊髓T11-S1区。脊髓中间神经元主要分布在腰骶脊髓背侧灰色连接区和副交感神经节前神经元区。与成年大鼠相比，青春期前大鼠在运动神经元所在的L5-S1区有更多的prv标记的脊髓中间神经元，但在脊髓的顶部分布相对较少。L3-L4内侧灰色腰椎丘脑神经元共定位PRV和GRP。在大脑中，在已知与男性性行为有关的区域，包括腹外侧髓质、下丘脑室旁核和内侧视前区，可以看到一致的标记。结论共同的脊髓和脑通路投射到大鼠的LA和BS肌肉，表明这些肌肉共同协调雄性性反射。与成人相比，青少年突触连接/神经元通路的数量可能存在差异。Dobberfuhl AD, Oti T, Sakamoto H, and Marson L.雄性大鼠提肛肌和腹侧球海绵肌中枢神经系统神经元的鉴定。中华性医学杂志2014；11:664-677。
IntroductionThe pelvic striated muscles play an important role in mediating erections and ejaculation, and together these muscles compose a tightly coordinated neuromuscular system that is androgen sensitive and sexually dimorphic.AimTo identify spinal and brains neurons involved in the control of the levator ani (LA) and bulbospongiosus (BS) in the male adult and preadolescent rat.MethodsRats were anesthetized, and the transsynaptic retrograde tracer pseudorabies virus (PRV) was injected into the LA muscle of adults or the ventral BS muscle in 30-day-old rats. After 3-5 days rats were sacrificed, and PRV-labeled neurons in the spinal cords and brains were identified using immunohistochemistry. The presence of gastrin-releasing peptide (GRP) in the lumbar spinal neurons was examined.Main Outcomes MeasuresThe location and number of PRV-labeled neurons in the spinal cord and brain and GRP colocalization in the lumbar spinal cord.ResultsPRV-labeled spinal interneurons were found distributed throughout T11-S1 of the spinal cord, subsequent to dorsal medial motoneuron infection. The majority of spinal interneurons were found in the lumbosacral spinal cord in the region of the dorsal gray commissure and parasympathetic preganglionic neurons. Preadolescent rats had more PRV-labeled spinal interneurons at L5-S1 where the motoneurons were located but relatively less spread rostrally in the spinal cord compared with adults. Lumbar spinothalmic neurons in medial gray of L3-L4 co-localized PRV and GRP. In the brain consistent labeling was seen in areas known to be involved in male sexual behavior including the ventrolateral medulla, hypothalamic paraventricular nucleus, and medial preoptic area.ConclusionCommon spinal and brain pathways project to the LA and BS muscles in the rat suggesting that these muscles act together to coordinate male sexual reflexes. Differences may exist in the amount of synaptic connections/neuronal pathways in adolescents compared with adults. Dobberfuhl AD, Oti T, Sakamoto H, and Marson L. Identification of CNS neurons innervating the levator ani and ventral bulbospongiosus muscles in male rats. J Sex Med 2014;11:664-677.