Relationship between endotoxin and prostaglandin (PGE2 and PGFM) concentrations and ovarian function in dairy cows with puerperal endometritis
Relationship between endotoxin and prostaglandin (PGE2 and PGFM) concentrations and ovarian function in dairy cows with puerperal endometritis
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DOI：
10.1016/s0378-4320(02)00248-8
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发表时间：
2003-04-15
影响因子：
2.2
通讯作者：
Ziecik, AJ
中科院分区：
文献类型：
--
作者：
Mateus, L;da Costa, LL;Ziecik, AJ
本文测定了14头产后子宫炎奶牛（轻度（n = 6）和重度（n = 8））血液中孕酮、13，14-二氢-15-酮-前列腺素F_（2 α）（PGFM）和内毒素的浓度，以及子宫液中前列腺素E_（2）、PGFM和内毒素的浓度。内毒素测定结果表明，重度子宫炎奶牛血浆PGFM（P < 0.01）、宫腔液PGE（2）和内毒素（P < 0.05）均显著高于轻度子宫炎奶牛。血浆和子宫液中PGFM、PGFM与PGE（2）、PGE（2）与子宫液中内毒素呈显著正相关（P < 0.05），轻度子宫炎奶牛中有1头血浆中有内毒素，重度子宫炎奶牛中有8头血浆中有内毒素。在产后1 - 12天（pp）观察到血浆内毒素峰值浓度（0.08-9.14内毒素单位/ml（EU/ml）），此后含量通常保持在0.1 EU/ml以下（检测最后一天：第27天pp）。在6头奶牛中观察到卵巢功能异常（4头发情期延长，2头产后排卵后黄体期延长）。检测了无胃奶牛血浆内毒素浓度，结果表明：（i）子宫液内毒素、PGE（2）和血浆PGFM浓度与子宫内膜炎的程度有关;（ii）内毒素从子宫吸收到血液中，主要发生在重度子宫内膜炎奶牛中;和（iii）子宫感染、内毒素产生和PP卵巢活性恢复之间存在关系。(C)2002 Elsevier Science B. V.保留所有权利。
Blood concentrations of progesterone, 13,14-dihydro-15-keto-prostaglandin F2alpha (PGFM) and endotoxin, and uterine fluid concentrations of prostaglandin E-2 (PGE(2)), PGFM and endotoxin were evaluated in 14 dairy cows with puerperal endometritis (mild (n = 6) and heavy (n = 8)). Endotoxin was measured using a quantitative kinetic assay.Cows with heavy endometritis had significantly higher concentrations of plasma PGFM (P < 0.01) and uterine fluid PGE(2) and endotoxin (P < 0.05) than cows with mild endometritis. Concentrations of PGFM in plasma and uterine fluid, of PGFM and PGE(2), and PGE(2) and endotoxin in uterine fluid were positively and significantly (P < 0.05) correlated.The presence of endotoxin in plasma was detected in one out of six mild and in eight out of eight heavy endometritis cows. Peak plasma endotoxin concentrations (0.08-9.14 endotoxin units/ml (EU/ml) were observed between 1 and 12 days postpartum (pp) and thereafter amounts generally remained below 0.1 EU/ml (last day of detection: Day 27 pp). Abnormal ovarian function was observed in six cows (four with prolonged anoestrus and two with long luteal phase after the first postpartum ovulation). Plasma endotoxin concentrations were detected in the anoestric cows.The results suggest that: (i) concentrations of uterine fluid endotoxin and PGE(2) and of plasma PGFM are related to the degree of endometritis; (ii) absorption of endotoxin from the uterus to the bloodstream occurs, mainly in heavy endometritis cows; and (iii) there is a relationship between uterine infection, endotoxin production and resumption of pp ovarian activity. (C) 2002 Elsevier Science B.V. All rights reserved.