Plasmodium malariae Prevalence and csp Gene Diversity, Kenya, 2014 and 2015
Plasmodium malariae Prevalence and csp Gene Diversity, Kenya, 2014 and 2015
复制标题
DOI：
10.3201/eid2304.161245
复制
发表时间：
2017-04-01
影响因子：
11.8
通讯作者：
Yan, Guiyun
中科院分区：
文献类型：
--
作者：
Lo, Eugenia;Nguyen, Kristie;Yan, Guiyun
在非洲，主要针对恶性疟原虫的控制规划不足以消除疟疾。为了进一步了解非洲疟疾疟原虫的流行情况和遗传变异性，我们在2014年6月至2015年8月期间检测了肯尼亚西部663名无症状者和245名有症状者的血液样本。疟疾疟原虫占无症状感染者的5.3%(35/663)，占临床病例的3.3%（8/245）。在无症状者中，71% (32/45)PCR检测的疟疾疟原虫感染镜检不出。显微镜的低灵敏度可能是由于疟原虫的寄生率明显较低。对非洲疟疾疟原虫环孢子子蛋白基因序列的分析表明，非洲疟疾疟原虫具有较高的遗传多样性，但在地理种群间没有明显的差异。我们的研究结果表明，疟疾疟原虫应该被纳入非洲的疟疾消除战略，并强调了在疟疾流行地区鉴定疟疾疟原虫的敏感和现场适用的方法的必要性。
In Africa, control programs that target primarily Plasmodium falciparum are inadequate for eliminating malaria. To learn more about prevalence and genetic variability of P. malariae in Africa, we examined blood samples from 663 asymptomatic and 245 symptomatic persons from western Kenya during June-August of 2014 and 2015. P. malariae accounted for 5.3% (35/663) of asymptomatic infections and 3.3% (8/245) of clinical cases. Among asymptomatic persons, 71% (32/45) of P. malariae infections detected by PCR were undetected by microscopy. The low sensitivity of microscopy probably results from the significantly lower parasitemia of P. malariae. Analyses of P. malariae circumsporozoite protein gene sequences revealed high genetic diversity among P. malariae in Africa, but no clear differentiation among geographic populations was observed. Our findings suggest that P. malariae should be included in the malaria elimination strategy in Africa and highlight the need for sensitive and field- applicable methods to identify P. malariae in malaria- endemic areas.