Atriopeptin release from the isolated perfused rabbit heart.
Atriopeptin release from the isolated perfused rabbit heart.
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从离体灌注兔心脏中释放心房肽。
DOI：
10.1016/0006-291x(84)91016-7
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发表时间：
1984
影响因子：
3.1
通讯作者：
P. Needleman
中科院分区：
文献类型：
--
作者：
M. Currie;D. Sukin;D. Geller;B. Cole;P. Needleman
哺乳动物心房提取物已被证明含有发挥利钠、利尿和平滑肌松弛作用的生物活性肽。这些提取物包括几种低分子量（<5，000 Mr）心房肽（心房肽），其在中心核心区域上显示出相同的序列，所述中心核心区域源自已被纯化和测序的高分子量肽（心房肽原）前体。本研究发现，兔心房提取物中含有高、低分子量的心房活性肽，而离体灌流兔心脏的冠状静脉流出液中仅含有低分子量的心房活性肽。这种胰蛋白酶不稳定活性导致兔主动脉和鸡直肠测定条的剂量依赖性松弛。用凝胶过滤色谱和反相HPLC分离生物活性表明心脏释放类似于心房肽素III的单一物质。无证据表明心房肽原从离体灌流的兔心脏中释放。我们认为，心房肽原是在心房蛋白水解加工成心房肽，这是随后的心房肽的释放形式。
Mammalian atrial extracts have been shown to contain bioactive peptides which exert natruiretic, diuretic, and smooth muscle relaxant effects. These extracts include several low molecular weight (< 5,000 Mr) atrial peptides (atriopeptins) which exhibit identical sequences over a central core region which are derived from the high molecular weight peptide (atriopeptigen) precursor which has been purified and sequenced. In the current study we found that extracts of rabbit atria possess both high and low molecular weight bioactive atrial peptides, however, the coronary venous effluent obtained from the isolated perfused rabbit heart only contained the low molecular weight peptide. This trypsin labile activity causes a dose-dependent relaxation of rabbit aorta and chicken rectum assay strips. Separation of the bioactivity with gel filtration chromatography and reversed phase HPLC indicates the heart releases a single substance similar to atriopeptin III. There was no evidence that atriopeptigen was released from the isolated perfused rabbit heart. We suggest that atriopeptigen is proteolytically processed in the atria to an atriopeptin which is subsequently the released form of the atrial peptide.
DOI：
10.1016/s0006-291x(84)80279-x
发表时间：
1984-03
影响因子：
3.1
作者：
K. Misono;H. Fukumi;R. Grammer;T. Inagami
通讯作者：
K. Misono;H. Fukumi;R. Grammer;T. Inagami