Environmental stress determines the colonization and impact of an endophytic fungus on invasive knotweed
Environmental stress determines the colonization and impact of an endophytic fungus on invasive knotweed
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DOI：
10.1007/s10530-022-02749-y
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发表时间：
2022-02-18
影响因子：
2.9
通讯作者：
Bossdorf，Oliver
中科院分区：
文献类型：
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作者：
Garnica，Sigisfredo;Liao，Zhiyong;Bossdorf，Oliver
越来越多的证据表明微生物在某些植物入侵中发挥着关键作用。一组多样化且分布广泛但鲜为人知的植物相关微生物是 Sebacinales 目真菌根内生菌。它们与欧洲的外来入侵虎杖（Reynoutriassp.）种群有关，但它们对入侵者的影响尚不清楚。我们利用最近分离的 Sebacinales 根内生真菌 Serendipita herbamans 来实验接种入侵虎杖，并研究不同环境条件下根部定植和对虎杖生长的影响。我们通过免疫荧光显微镜和 qPCR 验证了接种成功和真菌定植。我们发现S.赫巴曼在低营养和遮荫环境中强烈定殖入侵虎杖，但在干旱或良性条件下则较少。在低营养条件下，内生菌对植物生长有积极影响，而在遮荫条件下则相反。我们的研究表明根内生菌。赫巴曼具有定殖入侵虎杖细根并影响其生长的潜力，因此它也可以在自然种群中发挥作用。我们的结果还表明，内生真菌对植物的影响可能强烈依赖于环境，并且可能仅在应激环境条件下才可见。
There is increasing evidence that microbes play a key role in some plant invasions. A diverse and widespread but little understood group of plant-associated microbes are the fungal root endophytes of the order Sebacinales. They are associated with exotic populations of invasive knotweed (Reynoutriassp.) in Europe, but their effects on the invaders are unknown. We used the recently isolated Sebacinales root endophyte Serendipita herbamansto experimentally inoculate invasive knotweed and study root colonisation and effects on knotweed growth under different environmental conditions. We verified the inoculation success and fungal colonisation through immunofluorescence microscopy and qPCR. We found thatS. herbamansstrongly colonized invasive knotweed in low-nutrient and shade environments, but much less under drought or benign conditions. At low nutrients, the endophyte had a positive effect on plant growth, whereas the opposite was true under shaded conditions. Our study demonstrates that the root endophyteS. herbamanshas the potential to colonize invasive knotweed fine roots and impact its growth, and it could thus also play a role in natural populations. Our results also show that effects of fungal endophytes on plants can be strongly environment-dependent, and may only be visible under stressful environmental conditions.