Fatty acid shifts and metabolic activity changes of Schizochytrium sp S31 cultured on glycerol
Fatty acid shifts and metabolic activity changes of Schizochytrium sp S31 cultured on glycerol
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DOI：
10.1016/j.biortech.2013.05.030
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发表时间：
2013-08-01
影响因子：
11.4
通讯作者：
Wang, Xingguo
中科院分区：
文献类型：
--
作者：
Chang, Guifang;Luo, Zhenglin;Wang, Xingguo
采用逐步溶解氧策略，研究了Schizochytrium sp. S31在甘油上分批培养生产DHA。三个生长阶段分别为细胞生长、脂质积累和脂质周转。结果表明，脂肪酸（FA）在三个生长阶段的转移涉及甘油激酶（GK）、FAD（+）依赖性甘油-3-磷酸脱氢酶（FAD(+)-G-3-PDH）、苹果酸酶（ME）、ATP柠檬酸裂解酶（ACL）和NAD+依赖性异柠檬酸脱氢酶（NAD(+)-ICDH）活性的变化。Schizochytrium sp. S31的甘油异化是通过GK的磷酸化和随后的FAD(+)-G-3-PDH的氧化来实现的。该菌株的脂质积累是一个生长相关的过程，但可同化氮的消耗促进了脂质的积累。甘油的耗尽诱导了脂质转换阶段，短链脂肪酸优先降解并转化为无脂生物质（X-f），这与生物量中DHA含量的增加有关。(C) 2013 Elsevier Ltd.版权所有。
DHA production by Schizochytrium sp. S31 was studied in batch cultures on glycerol with stepwise dissolved oxygen strategy. Three growth stages were identified as cell growth, lipid accumulation and lipid turnover. It was revealed that fatty acid (FA) shifts during the three growth stages involved the activity changes of glycerol kinase (GK), FAD(+)-dependent glycerol-3-phosphate dehydrogenase (FAD(+)-G-3-PDH), malic enzyme (ME), ATP citrate lyase (ACL) and NAD+-dependent isocitrate dehydrogenase (NAD(+)-ICDH). Glycerol dissimilation in Schizochytrium sp. S31 was suggested via a phosphorylation by GK and a following oxidation by FAD(+)-G-3-PDH. Lipid accumulation of this strain was a growth-associated process, but the assimilable nitrogen depletion enhanced the accumulation of lipids. The exhaustion of glycerol induced the lipid turnover stage, where the short chain fatty acids were preferentially degraded and converted into lipid-free biomass (X-f) which was correlated to the increase of DHA content in biomass. (C) 2013 Elsevier Ltd. All rights reserved.