Non-Alu family interspersed repeats in human DNA and their transcriptional activity.
Non-Alu family interspersed repeats in human DNA and their transcriptional activity.
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非 Alu 家族散布在人类 DNA 中的重复序列及其转录活性。
DOI：
10.1093/nar/12.6.2669
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发表时间：
1984
影响因子：
14.9
通讯作者：
Leinwand,L
中科院分区：
文献类型：
--
作者：
Sun,L;Paulson,KE;Schmid,CW;Kadyk,L;Leinwand,L
随机选择的人类基因组克隆已经调查了非Alu家族散布重复序列的存在。四个这样的家庭的重复已被分离和特点的重复频率，散置，碱基序列，序列分歧，在体外RNA聚合酶III转录，延长转录在分离的细胞核，和在体内转录。四个重复序列家族中最丰富的两个家族对应于先前报道的重复序列家族，即Kpn I家族和poly（CA）。我们的结论是，大多数高度重复（> 50，000个拷贝）的人类散布重复序列已经被识别。本文还描述了两个丰度较低的重复序列家族。这些家族中的每一个在核RNA中的丰度定性地对应于它们的基因组重复频率。此外，每个重复序列家族的互补链近似对称地转录。这些重复序列在胞质RNA中的丰度在性质上低于核RNA。因此，这些重复序列的体内转录活性的大部分似乎是从其他启动子非特异性通读的
Randomly selected human genomic clones have been surveyed for the presence of non-Alu family interspersed repeats. Four such families of repeats have been isolated and characterized with respect to repetition frequency, interspersion, base sequence, sequence divergence,in vitroRNA polymerase III transcription, elongation of transcripts in isolated nuclei, andin vivotranscription. The two most abundant of the four families of repeats correspond to previously reported families of repeats, namely the Kpn I family and poly (CA). We conclude that most of the highly repetitive (> 50,000 copies) human interspersed repeats have already been identified. Two lower abundance repeats families are also described hereThe abundance with which each of these families is represented in nuclear RNA qualitatively corresponds to their genomic reiteration frequencies. Further, the complementary strands of each repeat family are approximately symmetrically transcribed. The abundance of these repeats in cytoplasmic RNA is qualitatively less than in nuclear RNA. The bulk of the in vivo transcriptional activity of these repeats thus appears to be nonspecific read through from other promoters