Soluble macromolecules from two Streptomyces strains with potent nematicidal activity against Meloidogyne incognita
Soluble macromolecules from two Streptomyces strains with potent nematicidal activity against Meloidogyne incognita
复制标题
来自两种链霉菌菌株的可溶性大分子，对南方根结线虫具有有效的杀线虫活性
DOI：
10.1016/j.rhisph.2022.100529
复制
发表时间：
2022-05-13
期刊：
影响因子：
3.7
通讯作者：
Liu, Yajun
中科院分区：
文献类型：
--
作者：
Hu, Qianru;Yang, Minmin;Liu, Yajun
放线菌是一类能产生杀线虫代谢产物的细菌，已被广泛报道。然而，大分子很少被描述。白灰链霉菌ND 41和S. fimicariusD 153对南方根结线虫的杀线虫活性分别为82.13%和86.96%。盆栽试验结果表明，在100 mL/盆的剂量下，30 d后，菌株ND 41和菌株D 153的生防效果分别达到68.89%和74.44%。这两株菌产生的活性代谢产物均为水溶性，ND 41的分子量在8000 Da以上，D153的分子量在1000-8000 Da之间。化学特性测试表明，这些活性代谢产物是含有大量糖和酚羟基化合物的极性聚合物。大多数杀线虫农药的不溶性限制了其剂型和应用范围。S. albogriseolus ND 41和S. Fimicarius D153水溶性大分子与土壤和根系相容性好，便于实际应用，是防治根结线虫的首选菌株。
Actinobacteria, which produce nematicidal metabolites, have been reported wildly. However, macromolecules were rarely described. The culture filtrates of Streptomyces albogriseolus ND41 and S. fimicarius D153 presented 82.13% and 86.96% nematicidal activity against Meloidogyne incognita after 12 h, respectively. A pot experiment highlighted that the biocontrol efficiency of strain ND41 reached 68.89% and that of strain D153 74.44% after 30 days at a dose of 100 mL per pot. The active metabolites produced by the two strains were hydrosoluble, with molecular weights above 8000 Da for ND41 and of 1000-8000 Da for D153. A chemical characteristic test indicated that these active metabolites were polar polymers that contained a large amount of sugar and phenolic hydroxyl compounds. The insoluble property of most nematicidal pesticide limits dosage form and range of application. Nematicidal metabolites from S. albogriseolus ND41 and S. fimicarius D153 would be preferred candidates for the control of root-knot nematodes, as hydrosoluble macromolecules have good compatibility to soil and root, and are convenient for practical application.