Analysis of B cell repertoire specific to the neutralizing epitopes of glycoprotein 120 in HIV-infected individuals.
Analysis of B cell repertoire specific to the neutralizing epitopes of glycoprotein 120 in HIV-infected individuals.
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对 HIV 感染者中糖蛋白 120 中和表位特异的 B 细胞库进行分析。
DOI：
10.1089/aid.1994.10.1629
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发表时间：
1994
影响因子：
1.5
通讯作者：
Kang,CY
中科院分区：
文献类型：
--
作者：
Hariharan,K;Nara,PL;Shabazz,LA;McCutchan,JA;Kang,CY
本研究描述了克隆型分析的中和抗gp 120抗体引起的HIV感染者的一组抗独特型单克隆抗体（抗Id单克隆抗体）。从80个HIV感染者在不同的临床阶段的HIV感染者的血清进行了测试，在ELISA中的19个抗Id单克隆抗体的反应性。抗独特型单克隆抗体与0 - 26%的血清反应。在抗CD 4位点抗体特异性的13种独特型中，4种在15%至20%的个体中表达，而抗V3抗体特异性的4种独特型中的2种在15%至26%的病例中表达。这些数据表明，每个HIV感染个体对给定的中和表位簇具有不同的B细胞库，并且某些克隆型比其他克隆型更普遍。为了将ELISA中的结合活性与抗gp 120特异性相关联，通过抗Id MAb-Sepharose亲和柱从代表性血清样品中分离独特型阳性抗体（Id+Abs）。在大多数情况下，分离的Id+ Ab的表位特异性和中和特性与用于产生抗Id MAbs的抗gp 120抗体的表位特异性和中和特性相关。我们建议，这些抗Id单克隆抗体可用于识别和测量中和抗gp 120抗体的定义特异性的HIV感染者的血清中，HIV疫苗接种的个人，并在HIV感染的母婴对。
This study describes the clonotypic analysis of neutralizing anti-gp120 antibodies elicited in HIV-infected individuals by a panel of anti-idiotype monoclonal antibodies (anti-Id MAbs). Sera from 80 HIV-infected individuals at various clinical stages of HIV-infection were tested for reactivity to 19 anti-Id MAbs in ELISA. Antiidiotype MAbs reacted with between 0 and 26% of sera. Among the 13 idiotypes specific for anti-CD4 site antibodies, 4 were expressed in 15 to 20% of individuals, whereas 2 of 4 idiotypes specific for anti-V3 antibodies were expressed in 15 to 26% of the cases. These data suggest that each HIV-infected individuals has a diverse B cell repertoire to a given neutralizing epitope cluster and that certain clonotypes are more prevalent than others. To correlate the binding activity in ELISA with anti-gp120 specificity, the idiotype-positive antibodies (Id+Abs) from representative serum samples were isolated by anti-Id MAb-Sepharose affinity columns. In most cases, the epitope specificity and the neutralizing properties of the isolated Id+Abs correlated with that of anti-gp120 antibodies used for the generation of anti-Id MAbs. We propose that these anti-Id MAbs may be used to identify and measure neutralizing anti-gp120 antibodies of defined specificity in the sera of HIV-infected individuals, HIV-vaccinated individuals, and in HIV-infected mother-infant pairs.