Detection of Pneumocystis carinii DNA by filtration of air.
Detection of Pneumocystis carinii DNA by filtration of air.
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通过空气过滤检测卡氏肺囊虫 DNA。
DOI：
10.3109/00365549609027173
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发表时间：
1996
影响因子：
--
通讯作者：
Ewert Linder
中科院分区：
文献类型：
--
作者：
M. Olsson;Antti Sukura;Lars;Ewert Linder
卡氏肺孢子虫在免疫抑制患者中引起的肺炎的高发病率使其成为非热带地理区域最重要的寄生虫。它最近被证明是一种真菌，但这种生物体的几个方面仍然知之甚少。与临床相关的一个主要问题是传播的性质，因此也是相关的预防问题。卡氏肺孢子虫的传播方式被认为是空气传播，但这是基于间接证据，传播阶段尚未确定。我们试图通过过滤含有卡氏肺孢子虫感染的Wistar大鼠的笼子附近的空气来捕获卡氏肺孢子虫。使用胸苷酸合成酶基因的巢式聚合酶链反应扩增来证明卡氏肺孢子虫，我们能够在这样的过滤器上证明卡氏肺孢子虫DNA。这有力地支持了肺孢子虫通过空气传播孢子的传播机制，并提出了一种分离和表征肺孢子虫的方法。
The high incidence of pneumonia caused by Pneumocystis carinii in immunosuppressed patients makes it the most important parasite in non-tropical geographical regions. It has recently been shown to be a fungus, but several aspects of this organism are still poorly understood. A major question of clinical relevance is the nature of transmission and, thereby, the related problem of prevention. The mode of P. carinii transmission is thought to be air, but this is based on circumstantial evidence, the transmissive stage has not been identified. We attempted to capture P. carinii by filtration of air in the vicinity of cages containing P. carinii infected Wistar rats. Using nested polymerase chain reaction amplification of the thymidylate synthase gene to demonstrate P. carinii we were able to demonstrate P. carinii DNA on such filters. This strongly supports the suggested mechanism of transmission of Pneumocystis by means of airborne spores and suggests an approach to their isolation and characterization.
空气中检测到卡氏肺囊虫。
DOI：
--
发表时间：
1994
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Bartlett,MS;Lee,CH;Lu,JJ;Bauer,NL;Bettz,JF;McLaughlin,GL;Smith,JW
通讯作者：
Smith,JW