Evaluation and comparison of three novel microbubbles: Enhancement of ultrasound-induced cell death and free radicals production
Evaluation and comparison of three novel microbubbles: Enhancement of ultrasound-induced cell death and free radicals production
复制标题
DOI：
10.1016/j.ultsonch.2008.10.003
复制
发表时间：
2009-03-01
影响因子：
8.4
通讯作者：
Kondo, Takashi
中科院分区：
文献类型：
--
作者：
Hassan, Mariame A.;Feril, Loreto B., Jr.;Kondo, Takashi
三种新型的脂质壳型微泡（MB），AS-0100，BG 6356 A和BG 6356 B，已被评估其对超声（US）诱导的细胞死亡和自由基产生的影响。采用先前研究和充分表征的US暴露条件，其中将人骨髓单核细胞淋巴瘤U937细胞暴露于1 MHz脉冲US束（0.3 W/cm（2），10%占空因数）1 min，有或没有MB。使用了三种不同浓度的每种MB。分别通过检查暴露后6 h的磷脂酰丝氨酸外化和计数活细胞来评估细胞凋亡和细胞溶解。采用电子顺磁共振（EPR）-自旋捕集法评价自由基产生和清除活性。结果表明，只有AS-0100和BG 6356 A能够增强超声诱导的细胞凋亡，主要是通过增加继发性坏死。细胞凋亡和细胞溶解似乎更多地依赖于振荡微球所施加的机械力，而自由基起着微不足道的作用。BG系列微球具有明显的清除活性。一般来说，尽管需要进一步优化，AS-0100和BG 6356 A似乎是有希望的，在需要US诱导细胞死亡的情况下作为替代品。(c)2008年由Elsevier B. V.出版。
Three novel lipid-shell-type microbubbles (MBs), AS-0100, BG6356A and BG6356B, have been evaluated for their impact on ultrasound (US)-induced cell death and free radicals production. Previously studied and well-characterized US exposure conditions were employed in which human myelomonocytic lymphoma U937 cells were exposed to 1 MHz pulsed US beam (0.3 W/cm(2), 10% duty factor) for 1 min with or without MBs. Three different concentrations of each MB were used. Apoptosis and cell lysis were assessed by examining phosphatidylserine externalization and by counting viable cells, respectively, 6 h post-exposure. Free radicals production and scavenging activities were evaluated using electron paramagnetic resonance (EPR)-spin trapping. The results showed that only AS-0100 and BG6356A were able to enhance the US-induced apoptosis, mainly by increasing the secondary necrosis. Apoptosis and cell lysis seemed to depend more on mechanical forces exerted by oscillating MBs while free radicals played a trivial role. BG series MBs exhibited pronounced scavenging activities. Generally, despite the need for further optimization, AS-0100 and BG6356A appear to be promising as adjuncts in cases where US-induced cell death is required. (c) 2008 Published by Elsevier B.V.