Antigens from Actinomyces Species and Their Value in Identification
Antigens from Actinomyces Species and Their Value in Identification
复制标题
放线菌抗原及其鉴定价值
DOI：
--
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
E. Fillery
中科院分区：
文献类型：
--
作者：
G. Bowden;J. Hardie;E. Fillery
放线菌具有复杂的抗原结构，包括中性细胞壁碳水化合物和一些含有带电抗原的多肽。壁碳水化合物可以从细胞壁或通过酸提取物和上清液培养液的DEAESephadex层析以相对纯的形式制备。带电抗原通常通过从柱分批洗脱而成组洗脱。细胞壁碳水化合物可以负责种特异性反应和交叉反应，并且菌株可以具有一个以上的细胞壁碳水化合物决定簇。带电荷的抗原也可以是种特异性的，但它们会引起一些交叉反应，特别是以色列曲霉血清型之间的交叉反应。菌株的高压灭菌提取物，对抗血清与细胞壁碳水化合物反应进行测试，可能在分离株的常规鉴定中有价值。
Actinomyces have a complex antigenic structure that includes neutral cell wall carbohydrate and some polypeptide containing charged antigens. Wall carbohydrate can be prepared in a relatively pure form from cell walls or by DEAE Sephadex chromatography of acid extracts and supernatant culture fluid. Charged antigens are generally eluted in groups by batch-elution from columns. Cell wall carbohydrate can be responsible for species-specific reactions and cross-reactions, and strains may possess more than one cell wall carbohydrate determinant. The charged antigens also can be species specific, but they cause some cross-reactions, particularly those between the A israelii serotypes. Autoclave extracts of strains, tested against antiserums reacting with cell wall carbohydrate, may be valuable in routine identification of isolates.