CUT & RUN to Profile Chromatin-Bound Proteins in Primary Mouse Neural Progenitor Cells.
CUT & RUN to Profile Chromatin-Bound Proteins in Primary Mouse Neural Progenitor Cells.
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DOI：
10.1007/978-1-0716-2847-8_8
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发表时间：
2023
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影响因子：
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通讯作者：
Peng,JamyC
中科院分区：
文献类型：
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作者：
Matsui,Yurika;Peng,JamyC
靶下切割和核酸酶释放（CUT & RUN）是一种创新的方法来分析组蛋白修饰和染色质结合的蛋白质全基因组。CUT & RUN具有两个明显的优势，即需要更少的细胞和在深度测序数据中提供强信噪比。在这里，我们描述了从小鼠胚胎脑的解离和分选、CUT & RUN和DNA文库制备到深度测序的工作流程。通过我们的工作流程，研究人员可以获得高质量的测序数据，通过使用少至100，000个神经祖细胞（NPC）来分析组蛋白和染色质相关蛋白。
Cleavage under targets and release using nuclease (CUT & RUN) is an innovative method to profile histone modifications and chromatin-bound proteins genome-wide. CUT & RUN offers two distinct advantages of requiring much fewer cells and providing strong signal-to-noise ratios in deep-sequencing data. Here, we describe a workflow starting from dissociation and sorting of mouse embryonic brains, CUT & RUN, and DNA library preparation to deep sequencing. With our workflow, researchers can obtain high-quality sequencing data to profile histones and chromatin-associated proteins by using as few as 100,000 neural progenitor cells (NPCs).