Morphological delimitation and molecular evidence for allopolyploidy in Collomia wilkenii (Polemoniaceae), a new species from northern Nevada
Morphological delimitation and molecular evidence for allopolyploidy in Collomia wilkenii (Polemoniaceae), a new species from northern Nevada
复制标题
DOI：
10.1600/036364406777585865
复制
发表时间：
2006-04-01
影响因子：
1
通讯作者：
Johnson, Robert L.
中科院分区：
文献类型：
--
作者：
Johnson, Leigh A.;Johnson, Robert L.
根据标准的有限的基因流证明通过标本聚集分析，和遗传学证据的障碍，基因交流与其最近的亲戚，我们描述了一个新种Polemoniaceae，Collomia wilkenii。Collomia wilkenii在某些特征上与Collomia tinctoria和Collomia linearis表面上相似，但经过检查，具有一致的独特特征组合，将其与这两个物种以及同域的Collomia renacta和Collomia tenella区分开来。这些特征包括花萼形态，花冠形态，雄蕊插入和发挥，花序簇中花的数量，腺毛和无腺毛的种类和分布的细节。叶绿体基因的比较DNA测序表明，Collomia wilkenii具有Collomia tenella的叶绿体基因组。核ITS序列在魏氏胶头菌中显示出线性胶头菌和柔嫩胶头菌之间的可加性，通过克隆该区域证实了这一模式。两个低拷贝的核基因座，idh-A和idh-B，表明这以前未描述的物种的异源多倍体起源。威肯氏Collomia wilkenii是北方内华达州的特有种，分布在该州的几个地方。
Under the criterion of limited homogenizing gene flow as evidenced through specimen aggregation analysis, and genetic evidence of a barrier to gene exchange with its closest relatives, we describe a new species in Polemoniaceae, Collomia wilkenii. Collomia wilkenii superficially resembles Collomia tinctoria and Collomia linearis in some features, but, upon examination, has consistent, unique character combinations that distinguish it from both species, as well as from sympatric Collomia renacta and Collomia tenella. These features include particulars of calyx morphology, corolla morphology, stamen insertion and exertion, numbers of flowers in inflorescence clusters, and the kinds and distribution of glandular and eglandular trichomes. Comparative DNA sequencing of chloroplast genes indicates Collomia wilkenii has the chloroplast genome of Collomia tenella. Nuclear ITS sequences show additivity in Collomia wilkenii between Collomia linearis and Collomia tenella, a pattern confirmed by cloning this region. Two low copy nuclear loci, idh-A and idh-B, indicate an allopolyploid origin of this previously undescribed species. Collomia wilkenii is endemic to northern Nevada, occurring in several locations across the breadth of the state.