AGGREGATION OF HUMAN-PLATELETS AND ADHESION OF STREPTOCOCCUS-SANGUIS
AGGREGATION OF HUMAN-PLATELETS AND ADHESION OF STREPTOCOCCUS-SANGUIS
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DOI：
10.1128/iai.39.3.1457-1469.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
CLAWSON, CC
中科院分区：
文献类型：
--
作者：
HERZBERG, MC;BRINTZENHOFE, KL;CLAWSON, CC
人类血小板选择性地结合或粘附在血链球菌和变形链球菌株上，并聚集成体外血栓的假设被研究。在一个简单的，定量的分析中，通过与血小板鬼孵育链球菌，使粘附从激活和聚集中解耦。黏附是由链球菌和血小板鬼魂上的蛋白酶敏感成分介导的，而不是细胞表面碳水化合物或右旋糖酐、血浆成分或二价阳离子。同样的链球菌也用标准聚集技术进行了研究。富血小板血浆被血链球菌的某些分离株激活和聚集。血小板鬼在Ca2+和血浆耗尽条件下选择性地结合同一菌株。清洗后活化的新鲜血小板；Ca2+必须恢复。聚集需要在Ca2+恢复的血浆中新鲜的血小板，并通过洗涤链球菌细胞壁诱导。透射电镜证实了这些结合和聚集实验中的反应。完整血螺表面的微纤维被鉴定出来。这些附属物将血链球菌与血小板结合在一起。各种血链球菌菌株对血小板鬼影或Ca2+和血浆耗尽的新鲜洗涤血小板的粘附选择性在所有供体中是相似的。因此，血小板结合位点在人群中广泛表达，不太可能是膜老化或制备的产物。由于血葡萄球菌与血小板的选择性粘附显然是聚集所必需的，因此某些血葡萄球菌菌株的配体功能定义受体可能存在于人血小板上。在血小板供者中，选择性和聚集反应率存在一定差异；可变性的含义需要进一步研究。这些相互作用可能有助于亚急性细菌性心内膜炎中植物性病变的发生和发展中的血小板增加。
The hypothesis that human platelets selectively bind or adhere to strains of S. Sanguis and S. mutans and aggregate, as a result, into an in vitro thrombus was investigated. Adhesion was uncoupled from activation and aggregation by incubating streptococci with platelet ghosts in a simple, quantitative assay. Adhesion was mediated by protease-sensitive components on the streptococci and platelet ghosts rather than cell surface carbohydrates or dextrans, plasma components or divalent cations. The same streptococci were also studied by standard aggregometry techniques. Platelet-rich plasma was activated and aggregated by certain isolates of S. sanguis. Platelet ghosts bound the same strains selectively under Ca2+ and plasma-depleted conditions. Fresh platelets activated after washing; Ca2+ had to be restored. Aggregation required fresh platelets in Ca2+-restored plasma and was inducible by washed streptococcal cell walls. These reactions in the binding and aggregometry assays were confirmed by transmission EM. Surface microfibrils on intact S. sanguis were identified. These appendages bound S. sanguis to platelets. The selectivity of adhesion of the various S. sanguis strains to platelet ghosts or Ca2+- and plasma-depleted fresh washed platelets was similar for all donors. Thus, the platelet binding site was expressed widely in the population and was unlikely to be an artifact of membrane aging or preparation. Since selective adhesion of S. sanguis to platelets was apparently required for aggregation, functionally defined receptors for ligands on certain strains of S. sanguis may be present on human platelets. Some differences in the selectivity and rate of the aggregation response were noted among platelet donors; the meaning of the variability requires further study. These interactions may contribute to platelet accretion in the initiation and development of vegetative lesions in subacute bacterial endocarditis.